Benschop K S M, Schinkel J, Minnaar R P, Pajkrt D, Spanjerberg L, Kraakman H C, Berkhout B, Zaaijer H L, Beld M G H M, Wolthers K C
Department of Clinical Virology, Academic Medical Center, Onze Lieve Vrouwe Gasthuis, Amsterdam, The Netherlands.
Clin Infect Dis. 2006 Jan 15;42(2):204-10. doi: 10.1086/498905. Epub 2005 Dec 12.
Human parechoviruses (HPeVs) are members of the family Picornaviridae and are classified into 3 known serotypes: HPeV1, HPeV2, and the recently identified HPeV3. HPeV1 and HPeV2 infections are most commonly associated with mild respiratory or gastrointestinal symptoms and occasionally with severe disease conditions, such as flaccid paralysis and encephalitis. HPeV3 infection has been associated with transient paralysis and neonatal infection and has until now only been reported in Japan and Canada.
Culture isolates considered to be enterovirus on the basis of cell culture but that were found to be enterovirus negative by 5' untranslated region reverse-transcriptase polymerase chain reaction (5'UTR RT-PCR) during the period December 2000 through January 2005 were selected. Isolates were tested by HPeV 5'UTR RT-PCR and were genotyped by sequencing the VP1 region. Phylogenetic analysis was performed, and the association with clinical symptoms was established.
Thirty-seven (12%) of the 303 isolates that tested positive for enterovirus by cell culture were in fact HPeV. The majority of the HPeV-positive isolates (n = 27) could be identified as HPeV1. The remaining 10 isolates, which were grown from samples obtained in 2001, 2002, and 2004, could be typed as the recently identified HPeV3. HPeV was exclusively detected in children aged < 3 years. Children infected with HPeV3 were significantly younger than children infected with HPeV1, and sepsis-like illness and central nervous system involvement were more frequently reported in children infected with HPeV3.
We report HPeV infections in young children during the period of 2000-2005 and show an association between HPeV3 infection and sepsis-like illness and central nervous system involvement in neonates.
人细小病毒（HPeVs）是小RNA病毒科的成员，分为3种已知血清型：HPeV1、HPeV2和最近鉴定出的HPeV3。HPeV1和HPeV2感染最常与轻度呼吸道或胃肠道症状相关，偶尔也与严重疾病有关，如弛缓性麻痹和脑炎。HPeV3感染与短暂性麻痹和新生儿感染有关，迄今为止仅在日本和加拿大有报道。
选择2000年12月至2005年1月期间基于细胞培养被认为是肠道病毒但通过5'非翻译区逆转录酶聚合酶链反应（5'UTR RT-PCR）检测为肠道病毒阴性的培养分离株。通过HPeV 5'UTR RT-PCR对分离株进行检测，并通过对VP1区域进行测序进行基因分型。进行系统发育分析，并确定与临床症状的关联。
303株通过细胞培养检测为肠道病毒阳性的分离株中，有37株（12%）实际上是HPeV。大多数HPeV阳性分离株（n = 27）可鉴定为HPeV1。其余10株分离株来自2001年、2002年和2004年获得的样本，可分型为最近鉴定出的HPeV3。HPeV仅在3岁以下儿童中检测到。感染HPeV3的儿童明显比感染HPeV1的儿童年龄小，感染HPeV3的儿童中败血症样疾病和中枢神经系统受累的报告更为频繁。
我们报告了2000 - 2005年期间幼儿中的HPeV感染，并显示HPeV3感染与败血症样疾病以及新生儿中枢神经系统受累之间存在关联。
