Yamashiro Sawako, Watanabe Naoki
Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto, Japan
Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto, Japan.
J Biochem. 2014 Nov;156(5):239-48. doi: 10.1093/jb/mvu053. Epub 2014 Sep 3.
The retrograde actin flow, continuous centripetal movement of the cell peripheral actin networks, is widely observed in adherent cells. The retrograde flow is believed to facilitate cell migration when linked to cell adhesion molecules. In this review, we summarize our current knowledge regarding the functional relationship between the retrograde actin flow and focal adhesions (FAs). We also introduce our recent study in which single-molecule speckle (SiMS) microscopy dissected the complex interactions between FAs and the local actin flow. FAs do not simply impede the actin flow, but actively attract and remodel the local actin network. Our findings provide a new insight into the mechanisms for protrusion and traction force generation at the cell leading edge. Furthermore, we discuss possible roles of the actin flow-FA interaction based on the accumulated knowledge and our SiMS study.
逆行肌动蛋白流，即细胞外周肌动蛋白网络持续向心运动，在贴壁细胞中广泛存在。当与细胞黏附分子相连时，逆行流被认为有助于细胞迁移。在本综述中，我们总结了目前关于逆行肌动蛋白流与黏着斑（FAs）之间功能关系的认识。我们还介绍了我们最近的一项研究，其中单分子斑点（SiMS）显微镜分析了黏着斑与局部肌动蛋白流之间的复杂相互作用。黏着斑并非简单地阻碍肌动蛋白流，而是积极吸引并重塑局部肌动蛋白网络。我们的研究结果为细胞前沿突起和牵引力产生的机制提供了新的见解。此外，我们基于积累的知识和我们的SiMS研究讨论了肌动蛋白流与黏着斑相互作用的可能作用。
