Department of Processing and Food Engineering, College of Agricultural Engineering and Technology, Punjab Agricultural University, Ludhiana, 141004 India.
J Food Sci Technol. 2014 Sep;51(9):1911-9. doi: 10.1007/s13197-012-0735-7. Epub 2012 May 27.
The respiratory behaviour of baby corn - whole and end-cut, under different temperatures and gas conditions was evaluated to study its respiratory dynamics based upon enzyme kinetics. The dependence of respiration rate on the O2 and CO2 concentrations has been described assuming the enzyme kinetics model for combined type of inhibition caused by CO2. Various respiratory parameters viz. maximal O2 consumption rate Michaelis-Menten constants, Inhibition constants under the specified environmental conditions of 5, 12.5, 20°C and 75% relative humidity (RH) were estimated using non-linear regression technique. The enzyme kinetics parameters were analyzed according to Arrhenius law to study their temperature dependence. Based upon the overall analysis, it was found that storage temperature had substantial affect on the partial pressures within the closed containers and subsequently the respiration rates. The concentration of O2 inside the container and surrounding temperature had synergistic effect on the rate of respiration and respiratory parameters. Though the inhibition by evolved CO2 was observed to be of combined type, it changed from predominantly competitive at 5°C to predominantly uncompetitive at 12.5 and 20°C. This study can be utilized for design of modified atmosphere packages for storage of fresh and minimally processed baby corn.
研究了不同温度和气体条件下整株和切段的玉米笋的呼吸行为，以基于酶动力学研究其呼吸动力学。根据 CO2 引起的综合抑制的酶动力学模型，描述了呼吸率对 O2 和 CO2 浓度的依赖性。使用非线性回归技术估算了各种呼吸参数，如最大 O2 消耗率、米氏常数、在 5、12.5、20°C 和 75%相对湿度 (RH) 等特定环境条件下的抑制常数。根据阿伦尼乌斯定律分析了酶动力学参数，以研究它们对温度的依赖性。基于全面分析，发现储存温度对密闭容器内的分压有很大影响，进而影响呼吸率。容器内的 O2 浓度和周围温度对呼吸率和呼吸参数有协同作用。尽管观察到 CO2 释放的抑制作用为综合类型，但它从 5°C 时的主要竞争性转变为 12.5°C 和 20°C 时的主要非竞争性。本研究可用于设计用于储存新鲜和轻度加工的玉米笋的改良气氛包装。
