Medicinal and Natural Product Research Laboratory, Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Matunga, Mumbai, India 400 019.
J Food Sci Technol. 2014 Sep;51(9):2251-5. doi: 10.1007/s13197-014-1270-5. Epub 2014 Feb 12.
The surge of interest in naturally occurring phytochemicals with high therapeutic potential has led to the discovery of many molecules, out of which naturally occuring coumarins such as marmelosin, umbelliferone and scopoletin present in Aegle marmelos (Bael) fruit shows good therapeutic potential. The aim of the present work is to develop and validate Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) method for simultaneous determination of marmelosin, umbelliferone and scopoletin in A. marmelos fruit extracts. The chromatographic separation was performed with isocratic elution of 55:45 (%, v/v) methanol-water containing 0.1 % acetic acid as mobile phase. The method used to analyse the extract of A. marmelos showed good resolution with retention time within 12 min. The relative concentrations of above phytoconstituent were determined in A. marmelos fruits. The method was found to give compact peaks for scopoletin, umbelliferone and marmelosin (Rt of 4.6, 6.5 and 11.3 min respectively) and were linear over the range 5-30 μg ml(-1) (R(2) = 0.9655), 2-10 μg ml(-1) (R(2) = 0.9964) and 2-10 μg ml(-1) (R(2) = 0.9862) respectively. The mean recoveries for marmelosin, umbelliferone and scopoletin at three concentrations were in the range of 98.8-102.9, 98.8-101.1 and 94.2-98.3 % respectively. The relative standard deviation of accuracy, precision and repeatability were within 2 %, indicating the method produced highly reproducible results. Therefore this simple, precise and accurate method enables simultaneous separation of this phytoconstituent and hence can be successfully applied in analysis and routine quality control of herbal material and formulation containing A. marmelos.
对具有高治疗潜力的天然植物化学物质的兴趣激增，导致发现了许多分子，其中，在 Aegle marmelos（Bael）果实中发现的天然香豆素，如 marmelosin、umbelliferone 和 scopoletin，具有良好的治疗潜力。本工作旨在开发和验证同时测定 A. marmelos 果实提取物中 marmelosin、umbelliferone 和 scopoletin 的反相高效液相色谱（RP-HPLC）方法。色谱分离采用等度洗脱，流动相为 55:45（%，v/v）甲醇-水，含 0.1%乙酸。用于分析 A. marmelos 提取物的方法显示出良好的分辨率，保留时间在 12 分钟内。测定了 A. marmelos 果实中上述植物成分的相对浓度。该方法对 scopoletin、umbelliferone 和 marmelosin 的峰形紧凑（Rt 分别为 4.6、6.5 和 11.3 分钟），在 5-30μgml(-1)（R(2) = 0.9655）、2-10μgml(-1)（R(2) = 0.9964）和 2-10μgml(-1)（R(2) = 0.9862）范围内呈线性。marmelosin、umbelliferone 和 scopoletin 在三个浓度下的平均回收率在 98.8-102.9%、98.8-101.1%和 94.2-98.3%之间。精密度、准确度和重复性的相对标准偏差均在 2%以内，表明该方法产生的结果高度重现。因此，这种简单、精确和准确的方法能够同时分离这些植物成分，因此可以成功应用于含有 A. marmelos 的草药材料和制剂的分析和常规质量控制。
