Baby Prathap Moothamadathil, Kumar Pramod, Kumar Rajesh, Jacob Sanu S, Rawat Dinesh, Binu V S, Karun Kalesh M
Department of Physiology, Melaka Manipal Medical College, Manipal campus, Manipal University, Karnataka, India.
Department of Plastic Surgery, King Abdul Aziz Specialist Hospital, Al Jouf, Saudi Arabia.
Indian J Plast Surg. 2014 May;47(2):242-8. doi: 10.4103/0970-0358.138961.
Blood volume measurement though important in management of critically ill-patients is not routinely estimated in clinical practice owing to labour intensive, intricate and time consuming nature of existing methods.
The aim was to compare blood volume estimations using trivalent chromium [(51)Cr(III)] and standard Evans blue dye (EBD) method in New Zealand white rabbit models and establish correction-factor (CF).
Blood volume estimation in 33 rabbits was carried out using EBD method and concentration determined using spectrophotometric assay followed by blood volume estimation using direct injection of (51)Cr(III). Twenty out of 33 rabbits were used to find CF by dividing blood volume estimation using EBD with blood volume estimation using (51)Cr(III). CF is validated in 13 rabbits by multiplying it with blood volume estimation values obtained using (51)Cr(III).
The mean circulating blood volume of 33 rabbits using EBD was 142.02 ± 22.77 ml or 65.76 ± 9.31 ml/kg and using (51)Cr(III) was estimated to be 195.66 ± 47.30 ml or 89.81 ± 17.88 ml/kg. The CF was found to be 0.77. The mean blood volume of 13 rabbits measured using EBD was 139.54 ± 27.19 ml or 66.33 ± 8.26 ml/kg and using (51)Cr(III) with CF was 152.73 ± 46.25 ml or 71.87 ± 13.81 ml/kg (P = 0.11).
The estimation of blood volume using (51)Cr(III) was comparable to standard EBD method using CF. With further research in this direction, we envisage human blood volume estimation using (51)Cr(III) to find its application in acute clinical settings.
尽管血容量测量在危重症患者的管理中很重要，但由于现有方法劳动强度大、操作复杂且耗时，在临床实践中并未常规进行估算。
旨在比较新西兰白兔模型中使用三价铬[（51）Cr（III）]和标准伊文思蓝染料（EBD）法估算血容量，并确定校正因子（CF）。
对33只兔子进行血容量估算，采用EBD法，用分光光度法测定浓度，然后通过直接注射（51）Cr（III）进行血容量估算。33只兔子中的20只用于通过将EBD法估算的血容量除以（51）Cr（III）法估算的血容量来确定CF。通过将CF与使用（51）Cr（III）获得的血容量估算值相乘，在13只兔子中对CF进行验证。
使用EBD法，33只兔子的平均循环血容量为142.02±22.77 ml或65.76±9.31 ml/kg，使用（51）Cr（III）法估算为195.66±47.30 ml或89.81±17.88 ml/kg。发现CF为0.77。使用EBD法测量的13只兔子的平均血容量为139.54±27.19 ml或66.33±8.26 ml/kg，使用（51）Cr（III）法并结合CF为152.73±46.25 ml或71.87±13.81 ml/kg（P = 0.11）。
使用（51）Cr（III）估算血容量与使用CF的标准EBD法相当。随着在这个方向上的进一步研究，我们设想使用（51）Cr（III）估算人体血容量，以在急性临床环境中找到其应用。
