Jahr J S, Lurie F, Xi S, Golkaryeh M, Kuznetsova O, Kullar R, Driessen B
Department of Anesthesiology and Pain Medicine, University of California-Davis, Sacramento, California, USA.
Anesth Analg. 2001 Mar;92(3):609-14. doi: 10.1097/00000539-200103000-00010.
Hemoglobin-based oxygen carriers (HBOC) may be ideal for monitoring circulating plasma volume (CV-P) and circulating blood volume (CV-B). We used an HBOC (Hemoglobin glutamer-200 [bovine], Oxyglobin; Biopure, Cambridge, MA) as an indicator for relative CV-B in the rabbit model. Accuracy of the technique was determined by comparison with the Evans blue dye (EBD) dilution technique in 19 anesthetized female New Zealand rabbits weighing 2.0 to 10.6 kg. The measurements were performed at baseline, after hemorrhage (1/3 of CV-B), normovolemic hemodilution (replacement of 1/3 CV-B by Hextend; Abbot Laboratories, North Chicago, IL), and hypervolemic hemodilution (additional infusion of Hextend(R) in a volume equal to 1/3 of CV-B). Hemoglobin concentration was measured by using a HemoCue photometer (HemoCue AB, Angelholm, Sweden). EBD concentration was analyzed by using linear regression to estimate Time 0 concentration; Time 0 was defined as EBD injection time. The difference between CV-P values determined by EBD and HBOC dilution was independent from the magnitude of the CV-P value. The relative bias was 1.29 mL, and the precision (one SD) was 2.82 mL. The difference did not reach statistical significance.
Circulating plasma and blood volumes can be accurately estimated by plasma hemoglobin concentration measurements by using hemoglobin-based oxygen carrier infusion.
基于血红蛋白的氧载体（HBOC）可能是监测循环血浆量（CV-P）和循环血量（CV-B）的理想选择。我们使用一种HBOC（血红蛋白谷氨酰胺-200[牛]，氧合血红蛋白；Biopure，马萨诸塞州剑桥）作为兔模型中相对CV-B的指标。通过与伊文思蓝染料（EBD）稀释技术比较，在19只体重2.0至10.6千克的麻醉雌性新西兰兔中确定该技术的准确性。测量在基线、出血后（CV-B的1/3）、等容血液稀释（用贺斯[Hextend]替代1/3 CV-B；雅培实验室，伊利诺伊州北芝加哥）和高容血液稀释（额外输注与1/3 CV-B体积相等的贺斯）时进行。使用HemoCue光度计（HemoCue AB，瑞典安吉尔霍尔姆）测量血红蛋白浓度。通过线性回归分析EBD浓度以估计0时刻浓度；0时刻定义为EBD注射时间。EBD和HBOC稀释法测定的CV-P值之间的差异与CV-P值大小无关。相对偏差为1.29毫升，精密度（一个标准差）为2.82毫升。差异未达到统计学显著性。
通过基于血红蛋白的氧载体输注测量血浆血红蛋白浓度，可以准确估计循环血浆量和血量。
