Sato B
Third Department of Internal Medicine, Osaka University Hospital, Japan.
Hum Cell. 1989 Sep;2(3):246-53.
Steroid hormone-responsive cell lines were clones from mouse mammary cancer (Shionogi Carcinoma 115) and Leydig cell tumor. SC-3 and SC-4 cells from Shionogi Carcinoma were androgen-responsive and -unresponsive in a serum-free medium, respectively. SC-3 cells secreted FGF-like growth factor as well as 24 K glycoprotein in response to androgen stimuli. B-1 and B-1F cells from mouse Leydig cell tumor were growth-stimulated in a serum-free medium by estrogen, androgen or retinoic acid. Transfection of ERE-TK-CAT gene into B-1F cells revealed that both estrogen and retinoic acid activated the CAT activity. In addition, the presence of corresponding receptors for steroid hormones or retinoic acid was demonstrated by hormone binding assays and/or Northern blot analysis. Thus, these serum-free culture systems seem to be very useful for analysing hormone action mechanisms in vitro.
类固醇激素反应性细胞系是从小鼠乳腺癌（狮王癌115）和睾丸间质细胞瘤克隆而来的。狮王癌的SC-3和SC-4细胞在无血清培养基中分别对雄激素有反应和无反应。SC-3细胞在雄激素刺激下分泌成纤维细胞生长因子样生长因子以及24K糖蛋白。小鼠睾丸间质细胞瘤的B-1和B-1F细胞在无血清培养基中受到雌激素、雄激素或视黄酸的生长刺激。将ERE-TK-CAT基因转染到B-1F细胞中表明，雌激素和视黄酸都激活了CAT活性。此外，通过激素结合试验和/或Northern印迹分析证明了存在类固醇激素或视黄酸的相应受体。因此，这些无血清培养系统似乎对体外分析激素作用机制非常有用。
