Nishizawa Y, Sato B, Nishii K, Kishimoto S, Matsumoto K
Third Department of Internal Medicine, Osaka University Hospital, Japan.
Cancer Res. 1989 Mar 15;49(6):1377-82.
We examined the effects of steroid hormones on the proliferation of transformed mouse Leydig cells (B-1) in serum-free culture condition. Among hormones examined, androgen as well as estrogen enhanced the cell proliferation rate. Hormone binding studies revealed that B-1 cells contained both androgen and estrogen receptors. In addition, androgen-enhanced cell growth was inhibited by antiandrogen, but not by antiestrogen, while estrogen-stimulated cell growth was suppressed by antiestrogen. However, the simultaneous addition of androgen and estrogen did not show an additive effect. Dose-response study on androgen-dependent cell growth revealed that relatively high concentrations (10(-7)-10(-6) M) of dihydrotestosterone were required to obtain the maximum response. This was at least partly explained by the finding that B-1 cells could metabolize dihydrotestosterone into the less active steroids. Finally, B-1 cells were found to grow more rapidly in normal than in castrated male mice. These results clearly indicate that the proliferation of B-1 cells is stimulated by both androgen and estrogen, which utilize the different receptor systems.
我们在无血清培养条件下研究了类固醇激素对转化的小鼠睾丸间质细胞（B-1）增殖的影响。在所检测的激素中，雄激素和雌激素均提高了细胞增殖率。激素结合研究表明，B-1细胞同时含有雄激素受体和雌激素受体。此外，抗雄激素可抑制雄激素增强的细胞生长，但抗雌激素则无此作用；而抗雌激素可抑制雌激素刺激的细胞生长。然而，同时添加雄激素和雌激素并未显示出相加效应。对雄激素依赖性细胞生长的剂量反应研究表明，需要相对较高浓度（10^(-7)-10^(-6) M）的二氢睾酮才能获得最大反应。这至少部分可以通过以下发现来解释：B-1细胞可将二氢睾酮代谢为活性较低的类固醇。最后，发现B-1细胞在正常雄性小鼠中比在去势雄性小鼠中生长得更快。这些结果清楚地表明，雄激素和雌激素均可刺激B-1细胞的增殖，且它们利用不同的受体系统。
