Kawai A, Ito S, Ozaki T, Inoue H, Tanabe G, Sato J
Department of Orthopaedic Surgery, Okayama University Medical School, Japan.
Hum Cell. 1989 Sep;2(3):283-9.
We investigated a new chemosensitivity test, MTT-hybrid assay, which was a hybrid of MTT colorimetric assay and double-layered soft agar colony assay, using human bone and soft tissue tumor cells. MTT formazan crystals produced by viable cells in the soft agar medium were solubilized by SDS at 60 degrees C. The absorbance (560 nm) is directly proportional to the cell number over a wide range. The absorbance increased in proportion to colonial growth of osteosarcoma cells, while it decreased in a human diploid cell strain in a few days. Drug sensitivity of tumor cells is supposed to be assessed without contaminating normal cells by MTT-hybrid assay in primary tumor samples. Good correlation of IC50 was observed between MTT-hybrid assay and colony assay. The MTT-hybrid assay shows potential value as a rapid predictive test for chemotherapeutic agents in an individual patient.
我们使用人骨和软组织肿瘤细胞，研究了一种新的化学敏感性测试方法——MTT杂交试验，它是MTT比色法和双层软琼脂集落试验的结合。软琼脂培养基中活细胞产生的MTT甲臜晶体在60℃下被SDS溶解。在很宽的范围内，吸光度（560nm）与细胞数量成正比。骨肉瘤细胞集落生长时吸光度增加，而人二倍体细胞株的吸光度在几天内下降。MTT杂交试验可在不污染原发性肿瘤样本中正常细胞的情况下评估肿瘤细胞的药物敏感性。MTT杂交试验与集落试验之间观察到IC50的良好相关性。MTT杂交试验作为对个体患者化疗药物的快速预测性测试显示出潜在价值。
