Li Chuang-Jun, Yuan Ling, Ji Teng-Fei, Yang Jian-Bo, Wang Ai-Guo, Su Ya-Lun
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China.
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China.
Fitoterapia. 2014 Dec;99:56-63. doi: 10.1016/j.fitote.2014.08.022. Epub 2014 Sep 2.
Allium cepa L. is one of the most widely cultivated and used plants. In addition to its bulb (onion), which is used as food in many cultures, the seeds of A. cepa L. are used as a traditional herbal medicine by the Uygur nationality in China to treat diarrhea and promote blood flow. In a bioactivity-screening, the ethanol extract of seeds of A. cepa L. showed inhibitory effects on protein tyrosine phosphatase 1B (PTP1B) enzyme, with 81.1% inhibition. Phytochemical investigation of the ethanol extract of red onion (Allium cepa L.) seeds led to the isolation of eight new furostanol saponins, named ceparosides E-L (1-8). Their structures were established using 1D and 2D NMR spectroscopy, mass spectrometry and chemical methods. Compounds 1-8 were screened for inhibitory effects on the PTP1B enzyme and cytotoxic activity against five human cells, including HCT-8, Bel-7402, BGC-823, A549 and A2780, but all were found to be inactive.
洋葱（Allium cepa L.）是种植和使用最为广泛的植物之一。除了其鳞茎（洋葱）在许多文化中用作食物外，洋葱种子在中国被维吾尔族用作传统草药来治疗腹泻和促进血液循环。在一项生物活性筛选中，洋葱种子的乙醇提取物对蛋白酪氨酸磷酸酶1B（PTP1B）酶显示出抑制作用，抑制率为81.1%。对红洋葱（Allium cepa L.）种子的乙醇提取物进行植物化学研究，分离出了8种新的呋甾烷醇皂苷，命名为洋葱皂苷E-L（1-8）。通过一维和二维核磁共振光谱、质谱和化学方法确定了它们的结构。对化合物1-8进行了对PTP1B酶的抑制作用以及对包括HCT-8、Bel-7402、BGC-823、A549和A2780在内的五种人类细胞的细胞毒性活性筛选，但发现所有化合物均无活性。
