Hatfield D, Rice M
Nucleic Acids Res. 1978 Oct;5(10):3491-502. doi: 10.1093/nar/5.10.3491.
Isoacceptors of Ala-, Arg-, Glu-, Gln-, Ile-, Leu-, Lys-, Ser-, Thr- and Val-tRNAs from wheat germ have been resolved by reverse phast chromatography. Codon recognition properties have been determined on isolated fractions of each of these aa-tRNAs and codon assignments have been made to a number of isoacceptors. Evolutionary changes which have occurred in patterns of codon recognition by isoacceptor aa-tRNAs in wheat germ and other organisms are discussed.
通过反向快速液相色谱法分离了来自小麦胚芽的丙氨酸、精氨酸、谷氨酸、谷氨酰胺、异亮氨酸、亮氨酸、赖氨酸、丝氨酸、苏氨酸和缬氨酸tRNA的同工受体。已对这些氨基酸tRNA的各个分离组分测定了密码子识别特性,并对许多同工受体进行了密码子指定。文中讨论了小麦胚芽和其他生物体中同工受体氨基酸tRNA在密码子识别模式上发生的进化变化。