Jones L D, Davies C R, Steel G M, Nuttall P A
Natural Environment Research Council, Institute of Virology, Oxford.
Med Vet Entomol. 1989 Apr;3(2):195-202. doi: 10.1111/j.1365-2915.1989.tb00498.x.
Experimental studies were undertaken to ascertain the vector potential of Rhipicephalus appendiculatus Neumann and Amblyomma variegatum Fabricius for Thogoto (THO) and Dhori (DHO) viruses, candidate members of the Orthomyxoviridae. In the first set of experiments, ticks were infected orally by feeding on viraemic hamsters. THO virus replicated in R. appendiculatus and A. variegatum, persisted trans-stadially in both tick species, and was transmitted to susceptible hosts during feeding. In contrast, both R. appendiculatus and A. variegatum were refractory to per os infection by DHO virus. In the second set of experiments, engorged R. appendiculatus and A. variegatum nymphs were parenterally inoculated with DHO virus. The virus persisted trans-stadially in both tick species and was transmitted by bite to susceptible hosts. These results indicate that the midgut acts as a barrier to per os infection of R. appendiculatus and A. variegatum by DHO virus. However, when this barrier is bypassed, i.e. by parenteral inoculation, both R. appendiculatus and A. variegatum can serve as efficient vectors of DHO virus.
开展了实验研究，以确定具尾扇头蜱（Rhipicephalus appendiculatus Neumann）和变异革蜱（Amblyomma variegatum Fabricius）作为正黏液病毒科候选成员索戈托（THO）病毒和多里（DHO）病毒的媒介潜能。在第一组实验中，蜱通过吸食患病毒血症的仓鼠而经口感染。THO病毒在具尾扇头蜱和变异革蜱中复制，在这两种蜱中都经变态持续存在，并在取食期间传播给易感宿主。相比之下，具尾扇头蜱和变异革蜱对DHO病毒的经口感染均具有抗性。在第二组实验中，对饱血的具尾扇头蜱若虫和变异革蜱若虫进行DHO病毒的非经口接种。该病毒在这两种蜱中都经变态持续存在，并通过叮咬传播给易感宿主。这些结果表明，中肠是DHO病毒对具尾扇头蜱和变异革蜱经口感染的屏障。然而，当绕过这一屏障时，即通过非经口接种，具尾扇头蜱和变异革蜱都可作为DHO病毒的有效媒介。
