Ouyang Zizhang, Cao Weiwei, Zhu Shaohua, Liu Xiaoping, Zhong Zhihua, Lai Xiangmao, Deng Huirong, Jiang Sheng, Wang Yan
Department of Pharmaceutical Sciences, Renmin Hospital of Qingyuan, The 5th Hospital Affiliated to Jinan University Qingyuan 511515, China.
School of Pharmaceutical Sciences, Sun Yat-Sen University Guangzhou 510006, China.
Int J Clin Exp Pathol. 2014 Jul 15;7(8):4587-95. eCollection 2014.
This study aims to explore the protective effect mechanism of 2-deoxy-D-glucose on nephrotoxicity of cyclosporin A in vivo.
Renal toxicity of SD rats model induced by CsA was established. Serum creatinine, blood urea nitrogen, urine NAG, GSH and MDA were determined and the histopathological changes of rat renal cortex were observed to explore the protective effects of 2-DG on CsA-induced nephrotoxicity.
Serum creatinine, BUN and urinary NAG of rats were significantly changed in experimental groups. Pathological results showed that there was obvious renal tubular injury in model group, however, the renal injury was significantly reduced in pre-treated with 2-DG.
2-DG had obvious protective effect on nephrotoxicity especially with high dose. This protective effect could be related to the reduction of ROS induced by CsA. However, 2-DG had no effect on the expression of RIP3.
本研究旨在探讨2-脱氧-D-葡萄糖对环孢素A体内肾毒性的保护作用机制。
建立环孢素A诱导的SD大鼠肾毒性模型。测定血清肌酐、血尿素氮、尿N-乙酰-β-D-氨基葡萄糖苷酶（NAG）、谷胱甘肽（GSH）和丙二醛（MDA），并观察大鼠肾皮质的组织病理学变化，以探讨2-脱氧-D-葡萄糖（2-DG）对环孢素A诱导的肾毒性的保护作用。
实验组大鼠血清肌酐、血尿素氮和尿NAG显著变化。病理结果显示，模型组有明显的肾小管损伤，然而，预先用2-DG处理后肾损伤明显减轻。
2-DG对肾毒性有明显的保护作用，尤其是高剂量时。这种保护作用可能与减少环孢素A诱导的活性氧（ROS）有关。然而，2-DG对受体相互作用蛋白3（RIP3）的表达没有影响。
