Suppr超能文献

结核分枝杆菌的烟酰胺酶/吡嗪酰胺酶形成由二硫键稳定的同型二聚体。

Nicotinamidase/pyrazinamidase of Mycobacterium tuberculosis forms homo-dimers stabilized by disulfide bonds.

作者信息

Rueda Daniel, Sheen Patricia, Gilman Robert H, Bueno Carlos, Santos Marco, Pando-Robles Victoria, Batista Cesar V, Zimic Mirko

机构信息

Unidad de Bioinformática y Biología Molecular, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru.

Department of International Health, Bloomberg School of Public Health, The Johns Hopkins University, USA.

出版信息

Tuberculosis (Edinb). 2014 Dec;94(6):644-8. doi: 10.1016/j.tube.2014.08.008. Epub 2014 Aug 24.

DOI:10.1016/j.tube.2014.08.008
PMID:25199451
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4258149/
Abstract

Recombinant wild-pyrazinamidase from H37Rv Mycobacterium tuberculosis was analyzed by gel electrophoresis under differential reducing conditions to evaluate its quaternary structure. PZAse was fractionated by size exclusion chromatography under non-reducing conditions. PZAse activity was measured and mass spectrometry analysis was performed to determine the identity of proteins by de novo sequencing and to determine the presence of disulfide bonds. This study confirmed that M. tuberculosis wild type PZAse was able to form homo-dimers in vitro. Homo-dimers showed a slightly lower specific PZAse activity compared to monomeric PZAse. PZAse dimers were dissociated into monomers in response to reducing conditions. Mass spectrometry analysis confirmed the existence of disulfide bonds (C72-C138 and C138-C138) stabilizing the quaternary structure of the PZAse homo-dimer.

摘要

对来自结核分枝杆菌H37Rv的重组野生型吡嗪酰胺酶在不同还原条件下进行凝胶电泳分析，以评估其四级结构。在非还原条件下通过尺寸排阻色谱法对PZAse进行分级分离。测量PZAse活性，并进行质谱分析，通过从头测序确定蛋白质的身份，并确定二硫键的存在。本研究证实结核分枝杆菌野生型PZAse能够在体外形成同型二聚体。与单体PZAse相比，同型二聚体的比PZAse活性略低。PZAse二聚体在还原条件下解离为单体。质谱分析证实存在稳定PZAse同型二聚体四级结构的二硫键（C72-C138和C138-C138）。

相似文献

1
Nicotinamidase/pyrazinamidase of Mycobacterium tuberculosis forms homo-dimers stabilized by disulfide bonds.结核分枝杆菌的烟酰胺酶/吡嗪酰胺酶形成由二硫键稳定的同型二聚体。
Tuberculosis (Edinb). 2014 Dec;94(6):644-8. doi: 10.1016/j.tube.2014.08.008. Epub 2014 Aug 24.
2
Metallochaperones Are Needed for Mycobacterium tuberculosis and Escherichia coli Nicotinamidase-Pyrazinamidase Activity.金属伴侣蛋白对于结核分枝杆菌和大肠杆菌烟酰胺酶-吡嗪酰胺酶活性是必需的。
J Bacteriol. 2020 Jan 2;202(2). doi: 10.1128/JB.00331-19.
3
Reduced pyrazinamidase activity and the natural resistance of Mycobacterium kansasii to the antituberculosis drug pyrazinamide.堪萨斯分枝杆菌的吡嗪酰胺酶活性降低及其对抗结核药物吡嗪酰胺的天然耐药性。
Antimicrob Agents Chemother. 1999 Mar;43(3):537-42. doi: 10.1128/AAC.43.3.537.
4
Mechanisms of pyrazinamide resistance in mycobacteria: importance of lack of uptake in addition to lack of pyrazinamidase activity.分枝杆菌中吡嗪酰胺耐药机制：除缺乏吡嗪酰胺酶活性外，摄取缺乏的重要性。
Microbiology (Reading). 1999 Jun;145 ( Pt 6):1359-1367. doi: 10.1099/13500872-145-6-1359.
5
Estimation of pyrazinamidase activity using a cell-free synthesis of pnca and its association with pyrazinamide susceptibility in .使用无细胞合成对硝基苯甲酸酯（pnca）估计吡嗪酰胺酶活性及其与吡嗪酰胺敏感性的关联。 （原文中“in.”后面似乎缺少内容）
Int J Mycobacteriol. 2018 Jan-Mar;7(1):16-25. doi: 10.4103/ijmy.ijmy_187_17.
6
Revelation of enzyme activity of mutant pyrazinamidases from Mycobacterium tuberculosis upon binding with various metals using quantum mechanical approach.利用量子力学方法揭示结核分枝杆菌突变吡嗪酰胺酶与各种金属结合时的酶活性。
Comput Biol Chem. 2019 Dec;83:107108. doi: 10.1016/j.compbiolchem.2019.107108. Epub 2019 Aug 16.
7
Expression of Mycobacterium smegmatis pyrazinamidase in Mycobacterium tuberculosis confers hypersensitivity to pyrazinamide and related amides.耻垢分枝杆菌吡嗪酰胺酶在结核分枝杆菌中的表达赋予了对吡嗪酰胺及相关酰胺的超敏性。
J Bacteriol. 2000 Oct;182(19):5479-85. doi: 10.1128/JB.182.19.5479-5485.2000.
8
Mutations in pncA, a gene encoding pyrazinamidase/nicotinamidase, cause resistance to the antituberculous drug pyrazinamide in tubercle bacillus.编码吡嗪酰胺酶/烟酰胺酶的基因pncA发生突变，会导致结核杆菌对抗结核药物吡嗪酰胺产生耐药性。
Nat Med. 1996 Jun;2(6):662-7. doi: 10.1038/nm0696-662.
9
[Nicotinamidase and the so-called pyrazinamidase in mycobacteria; the simultaneous occurrence of both activites (author's transl)].分枝杆菌中的烟酰胺酶和所谓的吡嗪酰胺酶；两种活性的同时存在（作者译）
Zentralbl Bakteriol Orig A. 1979 Jul;244(2-3):302-8.
10
Biochemical Characterization and Computational Identification of Mycobacterium tuberculosis Pyrazinamidase in Some Pyrazinamide-Resistant Isolates of Iran.伊朗部分耐吡嗪酰胺结核分枝杆菌分离株中结核分枝杆菌吡嗪酰胺酶的生化特性及计算鉴定
Protein J. 2015 Jun;34(3):181-92. doi: 10.1007/s10930-015-9610-5.

引用本文的文献

1
Metallochaperones Are Needed for Mycobacterium tuberculosis and Escherichia coli Nicotinamidase-Pyrazinamidase Activity.金属伴侣蛋白对于结核分枝杆菌和大肠杆菌烟酰胺酶-吡嗪酰胺酶活性是必需的。
J Bacteriol. 2020 Jan 2;202(2). doi: 10.1128/JB.00331-19.

本文引用的文献

1
Biochemical and mutational analysis of a novel nicotinamidase from Oceanobacillus iheyensis HTE831.海洋芽孢杆菌 HTE831 新型烟酰胺酶的生化和突变分析。
PLoS One. 2013;8(2):e56727. doi: 10.1371/journal.pone.0056727. Epub 2013 Feb 25.
2
The Leishmania nicotinamidase is essential for NAD+ production and parasite proliferation.利什曼原虫烟酰胺酶对于 NAD+ 的产生和寄生虫的增殖是必不可少的。
Mol Microbiol. 2011 Oct;82(1):21-38. doi: 10.1111/j.1365-2958.2011.07799.x. Epub 2011 Sep 1.
3
Structure-Activity relationship in mutated pyrazinamidases from Mycobacterium tuberculosis.结核分枝杆菌突变吡嗪酰胺酶的构效关系
Bioinformation. 2011;6(9):335-9. doi: 10.6026/97320630006335. Epub 2011 Jul 19.
4
Crystal structure of the pyrazinamidase of Mycobacterium tuberculosis: insights into natural and acquired resistance to pyrazinamide.结核分枝杆菌吡嗪酰胺酶的晶体结构：对天然和获得性吡嗪酰胺耐药性的深入了解。
PLoS One. 2011 Jan 24;6(1):e15785. doi: 10.1371/journal.pone.0015785.
5
Specificity and mechanism of Acinetobacter baumanii nicotinamidase: implications for activation of the front-line tuberculosis drug pyrazinamide.鲍曼不动杆菌烟酰胺酶的特异性和作用机制：对一线结核病药物吡嗪酰胺激活的影响
Angew Chem Int Ed Engl. 2009;48(48):9176-9. doi: 10.1002/anie.200903407.
6
Effect of pyrazinamidase activity on pyrazinamide resistance in Mycobacterium tuberculosis.吡嗪酰胺酶活性对结核分枝杆菌中吡嗪酰胺耐药性的影响。
Tuberculosis (Edinb). 2009 Mar;89(2):109-13. doi: 10.1016/j.tube.2009.01.004. Epub 2009 Feb 26.
7
The protonmotive force is required for maintaining ATP homeostasis and viability of hypoxic, nonreplicating Mycobacterium tuberculosis.质子动力对于维持缺氧、非复制性结核分枝杆菌的ATP稳态和生存能力是必需的。
Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11945-50. doi: 10.1073/pnas.0711697105. Epub 2008 Aug 12.
8
Biosynthesis and recycling of nicotinamide cofactors in mycobacterium tuberculosis. An essential role for NAD in nonreplicating bacilli.结核分枝杆菌中烟酰胺辅因子的生物合成与循环利用。NAD在非复制型杆菌中的重要作用。
J Biol Chem. 2008 Jul 11;283(28):19329-41. doi: 10.1074/jbc.M800694200. Epub 2008 May 19.
9
Characterization of Mycobacterium tuberculosis nicotinamidase/pyrazinamidase.结核分枝杆菌烟酰胺酶/吡嗪酰胺酶的特性分析
FEBS J. 2008 Feb;275(4):753-62. doi: 10.1111/j.1742-4658.2007.06241.x. Epub 2008 Jan 14.
10
In-gel digestion for mass spectrometric characterization of proteins and proteomes.用于蛋白质和蛋白质组质谱表征的胶内消化。
Nat Protoc. 2006;1(6):2856-60. doi: 10.1038/nprot.2006.468.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验