露水启发的基于呼吸的基因点突变检测，可裸眼可视化。
Dew inspired breathing-based detection of genetic point mutation visualized by naked eye.
作者信息
Xie Liping, Wang Tongzhou, Huang Tianqi, Hou Wei, Huang Guoliang, Du Yanan
机构信息
1] Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China [2] Institute of Molecular Medicine, College of Life and Health Science, Northeastern University, Shenyang 110004, China.
Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China.
出版信息
Sci Rep. 2014 Sep 9;4:6300. doi: 10.1038/srep06300.
A novel label-free method based on breathing-induced vapor condensation was developed for detection of genetic point mutation. The dew-inspired detection was realized by integration of target-induced DNA ligation with rolling circle amplification (RCA). The vapor condensation induced by breathing transduced the RCA-amplified variances in DNA contents into visible contrast. The image could be recorded by a cell phone for further or even remote analysis. This green assay offers a naked-eye-reading method potentially applied for point-of-care liver cancer diagnosis in resource-limited regions.
一种基于呼吸诱导蒸汽冷凝的新型无标记方法被开发用于检测基因点突变。通过将靶标诱导的DNA连接与滚环扩增（RCA）相结合，实现了受露水启发的检测。呼吸诱导的蒸汽冷凝将RCA扩增的DNA含量差异转化为可见对比度。该图像可以用手机记录以便进一步甚至远程分析。这种绿色检测方法提供了一种肉眼可读的方法，有可能应用于资源有限地区的即时护理肝癌诊断。
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