Pradko Andrey G, Litvinovskaya Raisa P, Sauchuk Alina L, Drach Svetlana V, Baranovsky Alexander V, Zhabinskii Vladimir N, Mirantsova Tatyana V, Khripach Vladimir A
Hemma-Test Ltd., Masherova St., 11, 220029 Minsk, Belarus.
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich St., 5/2, 220141 Minsk, Belarus.
Steroids. 2015 May;97:78-86. doi: 10.1016/j.steroids.2014.08.022. Epub 2014 Sep 6.
Starting from (22R,23R)-2α,3α,22,23,26-pentahydroxy-5α-cholestan-6-one 26-hemisuccinate, conjugates of 28-norcastasterone with horse radish peroxidase and bovine serum albumin were prepared. The latter conjugate was injected into rabbits; produced polyclonal antibodies were used to quantitate 6-keto-brassinosteroids. The newly developed analytical system was used in combination with two other immunoenzymatic assays for brassinosteroids to determine individual compounds of this series. In addition, a direct method of brassinosteroid analysis was proposed. It has the advantage of requiring no sample pretreatment steps such as extraction with organic solvents and chromatography.
从（22R，23R）-2α，3α，22，23，26-五羟基-5α-胆甾烷-6-酮26-半琥珀酸酯开始，制备了28-去甲油菜素甾酮与辣根过氧化物酶和牛血清白蛋白的缀合物。将后一种缀合物注射到兔子体内；产生的多克隆抗体用于定量6-酮油菜素甾类。新开发的分析系统与另外两种油菜素甾类免疫酶测定法结合使用，以测定该系列的单个化合物。此外，还提出了一种直接分析油菜素甾类的方法。它的优点是不需要诸如用有机溶剂萃取和色谱分析等样品预处理步骤。
