Kolomeichuk Liliya V, Murgan Ol'ga K, Danilova Elena D, Serafimovich Mariya V, Khripach Vladimir A, Litvinovskaya Raisa P, Sauchuk Alina L, Denisiuk Daria V, Zhabinskii Vladimir N, Kuznetsov Vladimir V, Efimova Marina V
Department of Plant Physiology, Biotechnology and Bioinformatics, Biological Institute, National Research Tomsk State University, Lenin Avenue 36, Tomsk 634050, Russia.
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich Street 5/2, 220084 Minsk, Belarus.
Plants (Basel). 2024 May 13;13(10):1345. doi: 10.3390/plants13101345.
The aim of this work was to study the ability of 28-homobrassinolide (HBL) and 28-homocastasterone (HCS) to increase the resistance of barley ( L.) plants to drought and to alter their endogenous brassinosteroid status. Germinated barley seeds were treated with 0.1 nM HBL or HCS solutions for two hours. A water deficit was created by stopping the watering of 7-day-old plants for the next two weeks. Plants responded to drought through growth inhibition, impaired water status, increased lipid peroxidation, differential effects on antioxidant enzymes, intense proline accumulation, altered expression of genes involved in metabolism, and decreased endogenous contents of hormones (28-homobrassinolide, B-ketones, and B-lactones). Pretreatment of plants with HBL reduced the inhibitory effect of drought on fresh and dry biomass accumulation and relative water content, whereas HCS partially reversed the negative effect of drought on fresh biomass accumulation, reduced the intensity of lipid peroxidation, and increased the osmotic potential. Compared with drought stress alone, pretreatment of plants with HCS or HBL followed by drought increased superoxide dismutase activity sevenfold or threefold and catalase activity (by 36%). The short-term action of HBL and HCS in subsequent drought conditions partially restored the endogenous B-ketone and B-lactone contents. Thus, the steroidal phytohormones HBL and HCS increased barley plant resistance to subsequent drought, showing some specificity of action.
这项工作的目的是研究28-高油菜素内酯（HBL）和28-高卡斯太甾酮（HCS）提高大麦（L.）植株抗旱性以及改变其内源油菜素甾醇状态的能力。将萌发的大麦种子用0.1 nM的HBL或HCS溶液处理两小时。通过在接下来的两周内停止对7日龄植株浇水来制造水分亏缺。植株对干旱的响应表现为生长受抑制、水分状况受损、脂质过氧化增加、对抗氧化酶的影响不同、脯氨酸大量积累、参与代谢的基因表达改变以及激素（28-高油菜素内酯、β-酮和β-内酯）的内源含量降低。用HBL预处理植株可降低干旱对鲜重和干重积累以及相对含水量的抑制作用，而HCS可部分逆转干旱对鲜重积累的负面影响，降低脂质过氧化强度，并增加渗透势。与单独的干旱胁迫相比，用HCS或HBL预处理植株后再进行干旱处理，超氧化物歧化酶活性提高了7倍或3倍，过氧化氢酶活性提高了36%。在随后的干旱条件下，HBL和HCS的短期作用部分恢复了内源β-酮和β-内酯的含量。因此，甾体类植物激素HBL和HCS提高了大麦植株对后续干旱的抗性，表现出一定的作用特异性。
