Hu Tingzhang, Yang Yongwei, Tan Lili, Yin Tieying, Wang Yazhou, Wang Guixue
Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.
Biomed Mater Eng. 2014;24(5):1837-49. doi: 10.3233/BME-140994.
Polysaccharides, such as alginates, are already being used as carriers for drug delivery. The physicochemical and biological properties of alginates may be affected via irradiation and thermal treatments. To explore and compare effects of two kinds of sterilization methods, gamma irradiation and moist heat, on sodium alginate (SA), physicochemical and biological properties of SA powder and solutions were investigated after sterilization. Human umbilical vein endothelial cells (HUVEC) was used to assess the cytotoxicity of the SA after sterilization. The research showed that 25 kGy gamma ray can effectively sterilize microorganism. Both gamma irradiation and moist heat hardly affect the native pH of SA. Compared to irradiation sterilization, moist heat sterilization showed smaller changes in intrinsic viscosity for all SA samples and lead to less glycosidic bond breaking of SA powders. The moist heat sterilization can cause the main chain scission and double bonds formation of the SA solutions. Cytotoxicity studies demonstrated that sterilized SA powers and SA solutions treated by gamma ray sterilization can increase the viability of HUVEC. However, SA solutions treated by moist heat sterilization were found to present severe cytotoxicity. The research results may provide interesting future advancements toward the development of SA-based products for biomedical applications.
多糖，如藻酸盐，已被用作药物递送的载体。藻酸盐的物理化学和生物学性质可能会受到辐照和热处理的影响。为了探索和比较两种灭菌方法，即γ辐照和湿热，对海藻酸钠（SA）的影响，研究了灭菌后SA粉末和溶液的物理化学和生物学性质。使用人脐静脉内皮细胞（HUVEC）评估灭菌后SA的细胞毒性。研究表明，25 kGy的γ射线可以有效杀灭微生物。γ辐照和湿热对SA的天然pH值几乎没有影响。与辐照灭菌相比，湿热灭菌对所有SA样品的特性粘度变化较小，并且导致SA粉末的糖苷键断裂较少。湿热灭菌会导致SA溶液的主链断裂和双键形成。细胞毒性研究表明，经γ射线灭菌处理的灭菌SA粉末和SA溶液可以提高HUVEC的活力。然而，发现经湿热灭菌处理的SA溶液具有严重的细胞毒性。研究结果可能为基于SA的生物医学应用产品的开发提供有趣的未来进展。
