Leo W J, McLoughlin A J, Malone D M
Department of Industrial Microbiology, University College Dublin, Belfield, Ireland.
Biotechnol Prog. 1990 Jan-Feb;6(1):51-3. doi: 10.1021/bp00001a008.
Aqueous sodium alginate solutions were subjected to various heat sterilization treatments. Sodium alginate powder was also treated by both gamma-irradiation and ethylene oxide sterilization. The effects of these treatments on the viscosities of sodium alginate solutions and both the diameter and strength of the beads formed in 0.1 M CaCl2 solutions were determined quantitatively. The viscosity of sodium alginate solutions and the gel strength of the calcium alginate beads decreased with increasing sterilization temperature while the bead diameters were found to increase. All these effects can be attributable to a reduction in the degree of polymerization of the alginate molecules as a result of the heat treatments. Ethylene oxide and gamma-irradiation treatments caused similar effects. Standard conditions for sterilization are necessary for comparative studies with alginate beads.
对海藻酸钠水溶液进行了各种热灭菌处理。海藻酸钠粉末也经过了伽马射线辐照和环氧乙烷灭菌处理。定量测定了这些处理对海藻酸钠溶液粘度以及在0.1M氯化钙溶液中形成的珠子的直径和强度的影响。海藻酸钠溶液的粘度和海藻酸钙珠子的凝胶强度随着灭菌温度的升高而降低，而珠子直径则增大。所有这些影响都可归因于热处理导致海藻酸盐分子聚合度降低。环氧乙烷和伽马射线辐照处理产生了类似的效果。对于海藻酸盐珠子的比较研究，灭菌的标准条件是必要的。
