O'Connell K P, Handelsman J
Department of Plant Pathology, University of Wisconsin, Madison 53706.
Mol Plant Microbe Interact. 1989 Jan-Feb;2(1):11-6.
Extracellular and intracellular neutral beta-1,2-linked D-glucan content was determined in a virulent, attachment-deficient mutants of Agrobacterium tumefaciens that map in the chvA locus. chvA mutants contained approximately the same amount of intracellular glucan as cells of the virulent control strain A759, but released into the culture medium only 2% of the glucan released by strain A759. Introduction of a cosmid carrying the wild-type chv region restored attachment and virulence and restored extracellular glucan production to chvA mutant A2505. Exogenous glucan did not enhance or inhibit attachment or tumorigenesis of the virulent control strain or the chvA or chvB mutants. Our results suggest that the chvA locus is involved in the export of glucan from the cell and that export may be required for tumorigenesis.
测定了根癌土壤杆菌的一个在chvA基因座定位的毒性、附着缺陷突变体中的细胞外和细胞内中性β-1,2-连接的D-葡聚糖含量。chvA突变体所含细胞内葡聚糖的量与毒性对照菌株A759的细胞大致相同，但释放到培养基中的葡聚糖仅为菌株A759释放量的2%。携带野生型chv区域的黏粒导入后恢复了chvA突变体A2505的附着和毒性，并恢复了细胞外葡聚糖的产生。外源性葡聚糖不会增强或抑制毒性对照菌株或chvA或chvB突变体的附着或肿瘤发生。我们的结果表明，chvA基因座参与葡聚糖从细胞中的输出，并且输出可能是肿瘤发生所必需的。
