2-连接的β-D-葡聚糖在根癌土壤杆菌毒力中的作用。
Role for 2-linked-beta-D-glucan in the virulence of Agrobacterium tumefaciens.
作者信息
Puvanesarajah V, Schell F M, Stacey G, Douglas C J, Nester E W
出版信息
J Bacteriol. 1985 Oct;164(1):102-6. doi: 10.1128/jb.164.1.102-106.1985.
Phenol-water cell extracts of virulent Agrobacterium tumefaciens A348 and several avirulent mutants with a reduced ability to attach to plant surfaces were examined. A low-molecular-weight 2-linked-beta-D-glucan was identified in the cell wall extracts of the virulent wild-type strain. Analyses of phenol-water extracts and culture filtrates of four mutant strains showed that the mutants did not produce any 2-linked-beta-D-glucan. When these mutants were complemented, the ability to produce the glucan described above was restored. These results suggest that there is a role for 2-linked-beta-D-glucans in the attachment of A. tumefaciens to plant cells. One avirulent, attachment-defective mutant retained its ability to produce the low-molecular-weight glucan. This mutation, however, mapped to a different transcriptional unit than the mutants deficient in the glucan described above. Thus, it appears that 2-linked-beta-D-glucan is only one component that may be necessary for attachment of A. tumefaciens to plant cell surfaces.
对强毒根癌土壤杆菌A348以及几种附着于植物表面能力降低的无毒突变体的酚-水细胞提取物进行了检测。在强毒野生型菌株的细胞壁提取物中鉴定出一种低分子量的2-连接-β-D-葡聚糖。对四种突变菌株的酚-水提取物和培养滤液的分析表明，这些突变体不产生任何2-连接-β-D-葡聚糖。当这些突变体得到互补时，产生上述葡聚糖的能力得以恢复。这些结果表明，2-连接-β-D-葡聚糖在根癌土壤杆菌附着于植物细胞的过程中发挥作用。一种无毒的、附着缺陷型突变体保留了产生低分子量葡聚糖的能力。然而，这种突变定位到一个与上述葡聚糖缺陷型突变体不同的转录单元。因此，看来2-连接-β-D-葡聚糖只是根癌土壤杆菌附着于植物细胞表面可能必需的一个组分。
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