Matton D P, Brisson N
Département de Biochimie, Université de Montréal, Québec, Canada.
Mol Plant Microbe Interact. 1989 Nov-Dec;2(6):325-31. doi: 10.1094/mpmi-2-325.
Treatment of potato tuber disks with arachidonic acid elicits the accumulation of several mRNAs. cDNA clones corresponding to two of these mRNAs were isolated and characterized. Nucleotide sequence analysis reveals that both clones (pSTH-2 and pSTH-21) contain an open-reading frame coding for a 155-amino acid polypeptide. The polypeptides encoded by the two clones differ by only six amino acids and show a high degree of similarity with PR protein sequences from pea (approximately 42%) and parsley (approximately 37%). mRNAs corresponding to the two potato cDNA clones also accumulate in Solanum chacoense and in tomato following elicitor treatment. Maximum accumulation of the mRNAs corresponding to the two cDNA clones is reached 24 hr after elicitor treatment of the tuber disks. pSTH-2-related mRNAs also accumulate in tubers after wounding or treatment with eicosapentaenoic acid and are detected in potato and tomato leaves treated with a Phytophthora infestans mycelium homogenate. The presence of these conserved genes in species from three plant families and the similarity of their induction pattern suggest an important function during the plant defense response.
用花生四烯酸处理马铃薯块茎圆盘会引发几种mRNA的积累。分离并鉴定了与其中两种mRNA对应的cDNA克隆。核苷酸序列分析表明，两个克隆（pSTH - 2和pSTH - 21）均包含一个编码155个氨基酸多肽的开放阅读框。两个克隆编码的多肽仅相差6个氨基酸，并且与豌豆（约42%）和欧芹（约37%）的病程相关蛋白序列具有高度相似性。在激发子处理后，与这两个马铃薯cDNA克隆对应的mRNA也在茄属查科茄和番茄中积累。在对块茎圆盘进行激发子处理后24小时，与这两个cDNA克隆对应的mRNA达到最大积累量。与pSTH - 2相关的mRNA在受伤或用二十碳五烯酸处理后的块茎中也会积累，并且在用致病疫霉菌丝体匀浆处理的马铃薯和番茄叶片中也能检测到。这三个植物科的物种中存在这些保守基因，以及它们诱导模式的相似性表明其在植物防御反应中具有重要功能。
