Collinge M, Boller T
Friedrich Miescher Institute, Basle, Switzerland.
Plant Mol Biol. 2001 Jul;46(5):521-9. doi: 10.1023/a:1010639225091.
To find out more about the interaction between potato and Phytophthora infestans at the molecular level, we screened for genes induced early after infection using mRNA differential display. Among the twenty cDNA clones recovered in the screen, two were found to represent plant genes whose transcript levels increased during infection of intact plants. These two genes differed strikingly in their response to wounding. Stprx2, a putative peroxidase, responded slowly and transiently to wounding, and its expression pattern was similar to that of gst1, a well-described pathogen-induced gene of potato. The second gene, StNAC, was induced rapidly and strongly after wounding but not systemically. Transcript levels reached a maximum after around 1 h and returned to basal levels after ca. 24 h. StNAC has strong similarity to the ATAF subfamily of NAC domain proteins, a large family of putative transcriptional activators. Arabidopsis ATAF1 and ATAF2 were also shown to be induced by wounding. This implies that the ATAF genes are not merely structurally similar but also share a conserved role in stress responses.
为了在分子水平上进一步了解马铃薯与致病疫霉之间的相互作用，我们利用mRNA差异显示技术筛选了感染后早期诱导表达的基因。在筛选得到的20个cDNA克隆中，有两个代表植物基因，其转录水平在完整植株感染期间有所增加。这两个基因对创伤的反应截然不同。Stprx2是一种假定的过氧化物酶，对创伤反应缓慢且短暂，其表达模式与gst1相似，gst1是一个已被充分描述的马铃薯病原体诱导基因。第二个基因StNAC在创伤后迅速强烈诱导，但没有系统性诱导。转录水平在约1小时后达到最大值，约24小时后恢复到基础水平。StNAC与NAC结构域蛋白的ATAF亚家族有很强的相似性，NAC结构域蛋白是一个假定的转录激活因子大家族。拟南芥ATAF1和ATAF2也被证明可被创伤诱导。这意味着ATAF基因不仅在结构上相似，而且在应激反应中也具有保守作用。
