Matton D P, Constabel P, Brisson N
Department of Biochemistry, Université de Montréal, Canada.
Plant Mol Biol. 1990 May;14(5):775-83. doi: 10.1007/BF00016510.
A cDNA clone corresponding to a mRNA that rapidly accumulates during the hypersensitive-like response induced by elicitor treatment of potato (Solanum tuberosum L.) tuber was characterized. The clone encodes a polypeptide (Mr = 41,097) having 83%-85% amino acid identity with known plant alcohol dehydrogenase sequences (ADH; EC 1.1.1.1). The identity of the clone was confirmed by measuring the ADH enzyme activity in extracts of Escherichia coli transformed with the cDNA clone. In potato tuber disks, a wide range of stresses, including treatment with fatty acid elicitors, salicylic acid, UV light and anaerobiosis, was shown to induce accumulation of Adh transcripts. In stems, a high constitutive level of Adh transcripts could be detected in 4-week old plants, but not in 8-week old plants. However, the mRNA could be induced to accumulate in stems of 8-week old plants by treatment with arachidonic acid elicitor or by anaerobiosis. Induction in leaves was also obtained during anaerobiosis and after treatment with a Phytophthora infestans mycelial homogenate.
对一个与信使核糖核酸（mRNA）对应的互补脱氧核糖核酸（cDNA）克隆进行了表征，该mRNA在激发子处理马铃薯（Solanum tuberosum L.）块茎诱导的类过敏反应期间迅速积累。该克隆编码一种多肽（分子量 = 41,097），与已知的植物乙醇脱氢酶序列（ADH；酶编号1.1.1.1）具有83%-85%的氨基酸同一性。通过测量用该cDNA克隆转化的大肠杆菌提取物中的ADH酶活性，证实了该克隆的同一性。在马铃薯块茎切片中，包括用脂肪酸激发子、水杨酸、紫外线和厌氧处理在内的多种胁迫被证明会诱导Adh转录本的积累。在茎中，在4周龄的植株中可检测到较高的Adh转录本组成水平，但在8周龄的植株中则检测不到。然而，通过用花生四烯酸激发子处理或厌氧处理，可诱导8周龄植株茎中的mRNA积累。在厌氧期间以及用致病疫霉菌丝体匀浆处理后，叶片中也出现了诱导现象。
