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Dlg1通过与LGN直接相互作用来控制神经上皮中平面纺锤体的方向。

Dlg1 controls planar spindle orientation in the neuroepithelium through direct interaction with LGN.

作者信息

Saadaoui Mehdi, Machicoane Mickaël, di Pietro Florencia, Etoc Fred, Echard Arnaud, Morin Xavier

机构信息

Institut de Biologie de l'École Normale Supérieure, Ecole Normale Supérieure, F-75005 Paris, France Institut National de la Santé et de la Recherche Medicale, U1024, F-75005 Paris, France Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8197, F-75005 Paris, France.

Membrane Traffic and Cell Division Laboratory, Institut Pasteur, F-75015 Paris, France Centre National de la Recherche Scientifique, Unité de Recherche Associée 2582, F-75015 Paris, France Cellule Pasteur-Université Pierre et Marie Curie, Université Pierre et Marie Curie, F-75015 Paris, France.

出版信息

J Cell Biol. 2014 Sep 15;206(6):707-17. doi: 10.1083/jcb.201405060. Epub 2014 Sep 8.

DOI:10.1083/jcb.201405060
PMID:25202028
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4164945/
Abstract

Oriented cell divisions are necessary for the development of epithelial structures. Mitotic spindle orientation requires the precise localization of force generators at the cell cortex via the evolutionarily conserved LGN complex. However, polarity cues acting upstream of this complex in vivo in the vertebrate epithelia remain unknown. In this paper, we show that Dlg1 is localized at the basolateral cell cortex during mitosis and is necessary for planar spindle orientation in the chick neuroepithelium. Live imaging revealed that Dlg1 is required for directed spindle movements during metaphase. Mechanistically, we show that direct interaction between Dlg1 and LGN promotes cortical localization of the LGN complex. Furthermore, in human cells dividing on adhesive micropatterns, homogenously localized Dlg1 recruited LGN to the mitotic cortex and was also necessary for proper spindle orientation. We propose that Dlg1 acts primarily to recruit LGN to the cortex and that Dlg1 localization may additionally provide instructive cues for spindle orientation.

摘要

定向细胞分裂对于上皮结构的发育至关重要。有丝分裂纺锤体定向需要通过进化上保守的LGN复合体将力产生器精确地定位在细胞皮层。然而，在脊椎动物上皮细胞体内，作用于该复合体上游的极性线索仍然未知。在本文中，我们表明Dlg1在有丝分裂期间定位于基底外侧细胞皮层，并且是鸡神经上皮中平面纺锤体定向所必需的。实时成像显示Dlg1是中期纺锤体定向运动所必需的。从机制上讲，我们表明Dlg1与LGN之间的直接相互作用促进了LGN复合体的皮层定位。此外，在粘附微图案上分裂的人类细胞中，均匀定位的Dlg1将LGN招募到有丝分裂皮层，并且对于正确的纺锤体定向也是必需的。我们提出Dlg1主要作用是将LGN招募到皮层，并且Dlg1的定位可能还为纺锤体定向提供指导性线索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8771/4164945/a64411484573/JCB_201405060R_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8771/4164945/b3dcd131ef2a/JCB_201405060_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8771/4164945/3867f0a17351/JCB_201405060R_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8771/4164945/c394af22b016/JCB_201405060_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8771/4164945/a64411484573/JCB_201405060R_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8771/4164945/b3dcd131ef2a/JCB_201405060_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8771/4164945/3867f0a17351/JCB_201405060R_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8771/4164945/c394af22b016/JCB_201405060_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8771/4164945/a64411484573/JCB_201405060R_Fig4.jpg

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