Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Stem Cell Research Center, K-STEMCELL Co., Ltd, Seoul, Republic of Korea.
Anticancer Res. 2014 Sep;34(9):4839-47.
BACKGROUND/AIM: Human mesenchymal stem cells (hMSCs) are thought to be one of the most reliable stem cell sources for a variety of cell therapies. This study investigated the anti-tumor effect of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) on EL4 murine T-cell lymphoma in vitro and in vivo.
The growth-inhibitory effect of hAT-MSCs on EL4 tumor cells was evaluated using a WST-1 cell proliferation assay. Cell-cycle arrest and apoptosis were investigated by flow cytometry and western blot. To evaluate an anti-tumor effect of hAT-MSCs on T-cell lymphoma in vivo, CM-DiI-labeled hAT-MSCs were circumtumorally injected in tumor-bearing nude mice, and tumor size was measured.
hAT-MSCs inhibited T-cell lymphoma growth by altering cell-cycle progression and inducing apoptosis in vitro. hAT-MSCs inhibited tumor growth in tumor-bearing nude mice and prolonged survival time. Immunofluorescence analysis showed that hAT-MSCs migrated to tumor sites.
hAT-MSCs suppress the growth of T-cell lymphoma, suggesting a therapeutic option for T-cell lymphoma.
背景/目的：人类间充质干细胞（hMSCs）被认为是各种细胞疗法最可靠的干细胞来源之一。本研究旨在探讨人脂肪组织来源的间充质干细胞（hAT-MSCs）对体外和体内 EL4 鼠 T 细胞淋巴瘤的抗肿瘤作用。
采用 WST-1 细胞增殖试验评估 hAT-MSCs 对 EL4 肿瘤细胞的生长抑制作用。通过流式细胞术和 Western blot 研究细胞周期阻滞和细胞凋亡。为了评估 hAT-MSCs 对 T 细胞淋巴瘤的体内抗肿瘤作用，将 CM-DiI 标记的 hAT-MSCs 肿瘤周注射到荷瘤裸鼠中，并测量肿瘤大小。
hAT-MSCs 通过改变细胞周期进程并诱导体外细胞凋亡来抑制 T 细胞淋巴瘤的生长。hAT-MSCs 抑制荷瘤裸鼠肿瘤生长并延长生存时间。免疫荧光分析表明 hAT-MSCs 迁移到肿瘤部位。
hAT-MSCs 抑制 T 细胞淋巴瘤的生长，提示其可能成为 T 细胞淋巴瘤的一种治疗选择。
