蛋白质组学鉴定二氢硫辛酰胺脱氢酶为子宫内膜癌患者自身抗体的靶标。
Proteomic identification of dihydrolipoamide dehydrogenase as a target of autoantibodies in patients with endometrial cancer.
机构信息
Department of Obstetrics and Gynecology, Nippon Medical School Hospital, Tokyo, Japan.
Division of Cellular Proteomics, Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
出版信息
Anticancer Res. 2014 Sep;34(9):5021-7.
BACKGROUND/AIM: Accumulating evidence shows that various types of cancers induce a specific immune response, resulting in the production of antibodies against self-components (autoantibodies). The aim of the present study was to identify antigens for autoantibodies in sera from endometrial cancer patients as novel diagnostic markers for the disease.
MATERIALS AND METHODS
The reactivity of individual sera from patients was examined by 2-dimensional (2-D) immunoblotting using HeLa cell lysates as antigens to identify autoantigens. ELISA was established to quantitatively measure autoantibody titer of patients' sera.
RESULTS
A mitochondrial protein, dihydrolipoamide dehydrogenase (DLD), was identified as an autoantigen specific to endometrial cancer patients. The levels of immunoglobulin (Ig)A but not IgG autoantibody to DLD were significantly increased in the sera of endometrial cancer patients.
CONCLUSION
IgA autoantibody against DLD could be a novel diagnostic marker for endometrial cancer.
背景/目的：越来越多的证据表明，各种类型的癌症会引发特定的免疫反应，导致针对自身成分（自身抗体）的抗体产生。本研究旨在鉴定子宫内膜癌患者血清中的自身抗体抗原，作为该疾病的新型诊断标志物。
材料与方法
使用 HeLa 细胞裂解物作为抗原，通过二维（2-D）免疫印迹检测个体血清的反应性，以鉴定自身抗原。建立 ELISA 定量测量患者血清中自身抗体滴度。
结果
一种线粒体蛋白二氢硫辛酰胺脱氢酶（DLD）被鉴定为子宫内膜癌患者特有的自身抗原。子宫内膜癌患者血清中的免疫球蛋白（Ig）A 而非 IgG 自身抗体对 DLD 的水平显著升高。
结论
针对 DLD 的 IgA 自身抗体可能是子宫内膜癌的一种新型诊断标志物。
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