在山榄科植物山榄属的研究中开发微卫星标记及其在山榄属银果榄中的可转移性。
Development of microsatellite markers in Cratylia mollis and their transferability to C. argentea (Fabaceae).
机构信息
Departamento Ciências Biológicas, Universidade Estadual de Feira de Santana (UEFS), Av. Transnordestina s.n., 44036-900 Feira de Santana, Bahia, Brazil.
出版信息
Appl Plant Sci. 2013 Oct 4;1(10). doi: 10.3732/apps.1300015. eCollection 2013 Oct.
PREMISE OF THE STUDY
This work aimed to develop microsatellite markers for Cratylia mollis as tools to assess its genetic diversity and structure and to evaluate their potential cross-amplification in related species. •
METHODS AND RESULTS
Microsatellite markers were developed using a microsatellite-enriched library and an intersimple sequence repeat library. From a set of 19 markers, 12 microsatellite loci were polymorphic and presented considerable variation in allele number (2-11), expected heterozygosity (0.226-0.883), and polymorphism information content per locus (0.212-0.870). Cross-amplification in C. argentea was successful in 16 loci, 12 of which were polymorphic (2-10 alleles). •
CONCLUSIONS
The polymorphism of this set of microsatellite markers for C. mollis, as well as their successful cross-amplification in C. intermedia and C. bahiensis and their transferability to C. argentea, supports their use in future comparative studies to understand the mechanism involved in population divergence and speciation in the genus.
研究前提
本研究旨在开发用于评估 Cratylia mollis 遗传多样性和结构的微卫星标记，并评估其在相关物种中的潜在交叉扩增。
方法和结果
使用微卫星富集文库和简单重复序列间间插重复（ISSR）文库开发微卫星标记。从一组 19 个标记中，12 个微卫星位点表现出多态性，等位基因数（2-11）、预期杂合度（0.226-0.883）和每个位点的多态信息含量（0.212-0.870）有显著差异。在 C. argentea 中成功扩增了 16 个位点，其中 12 个具有多态性（2-10 个等位基因）。
结论
C. mollis 这套微卫星标记的多态性，以及在 C. intermedia 和 C. bahiensis 中的成功交叉扩增，并可转移到 C. argentea 中，支持在未来的比较研究中使用这些标记来理解该属中种群分化和物种形成的机制。
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