金缕梅科微卫星标记的鉴定与开发
Identification and development of microsatellite markers in (Hamamelidaceae).
作者信息
Yin Qianyi, Huang Cuiying, Huang Yanshuang, Chen Sufang, Ye Huagu, Fan Qiang, Liao Wenbo
机构信息
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources Sun Yat-sen University Guangzhou 510275 People's Republic of China.
出版信息
Appl Plant Sci. 2018 Oct 23;6(10):e01189. doi: 10.1002/aps3.1189. eCollection 2018 Oct.
PREMISE OF THE STUDY
(Hamamelidaceae) is a Tertiary relict species endemic to southern China. Polymorphic microsatellite markers were developed to reveal the genetic diversity of this species.
METHODS AND RESULTS
The genome of was sequenced and de novo assembled into 642,351 contigs. A total of 72,097 paired primers were successfully designed from 80,282 simple sequence repeat (SSR) markers identified in 63,419 contigs. PCR amplification showed that 96 of the 136 synthesized primers could be successfully amplified, and 22 demonstrated polymorphism. The mean number of alleles, levels of observed heterozygosity, and levels of expected heterozygosity were 4.602 ± 0.140, 0.632 ± 0.020, and 0.696 ± 0.010, respectively. The majority of the 96 primer pairs could be amplified in at least one other Hamamelidaceae species, including (60), (39), (24), and (24).
CONCLUSIONS
These microsatellite loci provide abundant genomic SSR markers to evaluate genetic diversity of this woody ornamental plant.
研究前提
（金缕梅科）是中国南方特有的第三纪残遗物种。开发了多态性微卫星标记以揭示该物种的遗传多样性。
方法与结果
对（该物种）的基因组进行测序并从头组装成642,351个重叠群。从63,419个重叠群中鉴定出的80,282个简单序列重复（SSR）标记中成功设计了总共72,097对引物。PCR扩增表明，136个合成引物中有96个能够成功扩增，22个表现出多态性。等位基因的平均数、观察到的杂合度水平和预期的杂合度水平分别为4.602±0.140、0.632±0.020和0.696±0.010。96对引物中的大多数至少能在其他一种金缕梅科物种中扩增，包括（具体物种1）（60种）、（具体物种2）（39种）、（具体物种3）（24种）和（具体物种4）（24种）。
结论
这些微卫星位点提供了丰富的基因组SSR标记，用于评估这种木本观赏植物的遗传多样性。
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