• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Identification and development of microsatellite markers in (Hamamelidaceae).金缕梅科微卫星标记的鉴定与开发
Appl Plant Sci. 2018 Oct 23;6(10):e01189. doi: 10.1002/aps3.1189. eCollection 2018 Oct.
2
Identification and development of microsatellite (SSRs) makers of Exbucklandia (HAMAMELIDACEAE) by high-throughput sequencing.利用高通量测序技术鉴定和开发榆叶梅属（Hamamelidaceae）微卫星（SSR）标记。
Mol Biol Rep. 2019 Jun;46(3):3381-3386. doi: 10.1007/s11033-019-04800-z. Epub 2019 Apr 15.
3
Development and characterization of genomic microsatellite markers in the tree species, Rhodoleia championii, R. parvipetala, and R. forrestii (Hamamelidaceae).开发和鉴定木荷属（金缕梅科）三个树种的基因组微卫星标记：红花荷、小花红花荷和罗浮木。
Mol Biol Rep. 2019 Dec;46(6):6547-6556. doi: 10.1007/s11033-019-05106-w. Epub 2019 Oct 3.
4
Isolation and identification of EST-SSR markers in (Hamamelidaceae).金缕梅科（Hamamelidaceae）中EST-SSR标记的分离与鉴定。
Appl Plant Sci. 2016 Oct 14;4(10). doi: 10.3732/apps.1600064. eCollection 2016 Oct.
5
Development of microsatellites from Fothergilla ×intermedia (Hamamelidaceae) and cross transfer to four other genera within Hamamelidaceae.来自杂种福氏木（金缕梅科）的微卫星开发及向金缕梅科其他四个属的交叉转移。
Appl Plant Sci. 2015 Apr 7;3(4). doi: 10.3732/apps.1400123. eCollection 2015 Apr.
6
Development and characterization of genomic SSR markers for (Pentaphylacaceae).五列木科基因组SSR标记的开发与特征分析
Appl Plant Sci. 2017 Oct 26;5(10). doi: 10.3732/apps.1700086. eCollection 2017 Oct.
7
Novel microsatellite markers for Distylium lepidotum (Hamamelidaceae) endemic to the Ogasawara Islands.小笠原群岛特有的鳞毛蚊母树（金缕梅科）的新型微卫星标记。
BMC Res Notes. 2016 Jul 2;9:332. doi: 10.1186/s13104-016-2137-9.
8
Characterization and development of EST-derived SSR markers in (Hamamelidaceae).金缕梅科 EST 衍生 SSR 标记的特征分析与开发
Appl Plant Sci. 2017 Nov 14;5(11). doi: 10.3732/apps.1700080. eCollection 2017 Nov.
9
Isolation and identification of EST-SSR markers in (Ixonanthaceae).金丝桃科EST-SSR标记的分离与鉴定
Appl Plant Sci. 2019 May 10;7(5):e01246. doi: 10.1002/aps3.1246. eCollection 2019 May.
10
Mining and characterization of novel EST-SSR markers of Parrotia subaequalis (Hamamelidaceae) from the first Illumina-based transcriptome datasets.从首个基于 Illumina 的转录组数据集挖掘和表征珙桐（金缕梅科）的新型 EST-SSR 标记。
PLoS One. 2019 May 6;14(5):e0215874. doi: 10.1371/journal.pone.0215874. eCollection 2019.

引用本文的文献

1
Development and characterization of genomic microsatellite markers in the tree species, Rhodoleia championii, R. parvipetala, and R. forrestii (Hamamelidaceae).开发和鉴定木荷属（金缕梅科）三个树种的基因组微卫星标记：红花荷、小花红花荷和罗浮木。
Mol Biol Rep. 2019 Dec;46(6):6547-6556. doi: 10.1007/s11033-019-05106-w. Epub 2019 Oct 3.
2
Identification and development of microsatellite (SSRs) makers of Exbucklandia (HAMAMELIDACEAE) by high-throughput sequencing.利用高通量测序技术鉴定和开发榆叶梅属（Hamamelidaceae）微卫星（SSR）标记。
Mol Biol Rep. 2019 Jun;46(3):3381-3386. doi: 10.1007/s11033-019-04800-z. Epub 2019 Apr 15.

本文引用的文献

1
Characterization and development of EST-derived SSR markers in (Hamamelidaceae).金缕梅科 EST 衍生 SSR 标记的特征分析与开发
Appl Plant Sci. 2017 Nov 14;5(11). doi: 10.3732/apps.1700080. eCollection 2017 Nov.
2
Isolation and identification of EST-SSR markers in (Hamamelidaceae).金缕梅科（Hamamelidaceae）中EST-SSR标记的分离与鉴定。
Appl Plant Sci. 2016 Oct 14;4(10). doi: 10.3732/apps.1600064. eCollection 2016 Oct.
3
Novel microsatellite markers for Distylium lepidotum (Hamamelidaceae) endemic to the Ogasawara Islands.小笠原群岛特有的鳞毛蚊母树（金缕梅科）的新型微卫星标记。
BMC Res Notes. 2016 Jul 2;9:332. doi: 10.1186/s13104-016-2137-9.
4
Development of microsatellites from Fothergilla ×intermedia (Hamamelidaceae) and cross transfer to four other genera within Hamamelidaceae.来自杂种福氏木（金缕梅科）的微卫星开发及向金缕梅科其他四个属的交叉转移。
Appl Plant Sci. 2015 Apr 7;3(4). doi: 10.3732/apps.1400123. eCollection 2015 Apr.
5
Development and characterization of microsatellite markers from the transcriptome of Firmiana danxiaensis (Malvaceae s.l.).从 Firmiana danxiaensis（Malvaceae s.l.）转录组中开发和表征微卫星标记。
Appl Plant Sci. 2013 Nov 28;1(12). doi: 10.3732/apps.1300047. eCollection 2013 Dec.
6
GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research--an update.GenAlEx 6.5：Excel 中的遗传分析。用于教学和研究的种群遗传软件--更新。
Bioinformatics. 2012 Oct 1;28(19):2537-9. doi: 10.1093/bioinformatics/bts460. Epub 2012 Jul 20.
7
NGS QC Toolkit: a toolkit for quality control of next generation sequencing data.NGS QC 工具包：下一代测序数据质量控制工具包。
PLoS One. 2012;7(2):e30619. doi: 10.1371/journal.pone.0030619. Epub 2012 Feb 1.
8
De novo bacterial genome sequencing: millions of very short reads assembled on a desktop computer.从头开始的细菌基因组测序：在台式计算机上组装数百万条非常短的读段。
Genome Res. 2008 May;18(5):802-9. doi: 10.1101/gr.072033.107. Epub 2008 Mar 10.
9
Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.).利用EST数据库开发和鉴定大麦（Hordeum vulgare L.）基因衍生的SSR标记。
Theor Appl Genet. 2003 Feb;106(3):411-22. doi: 10.1007/s00122-002-1031-0. Epub 2002 Sep 14.
10
Primer3 on the WWW for general users and for biologist programmers.万维网上面向普通用户和生物学家程序员的Primer3。
Methods Mol Biol. 2000;132:365-86. doi: 10.1385/1-59259-192-2:365.

金缕梅科微卫星标记的鉴定与开发

Identification and development of microsatellite markers in (Hamamelidaceae).

作者信息

Yin Qianyi, Huang Cuiying, Huang Yanshuang, Chen Sufang, Ye Huagu, Fan Qiang, Liao Wenbo

机构信息

State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources Sun Yat-sen University Guangzhou 510275 People's Republic of China.

出版信息

Appl Plant Sci. 2018 Oct 23;6(10):e01189. doi: 10.1002/aps3.1189. eCollection 2018 Oct.

DOI:10.1002/aps3.1189
PMID:30386715
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6201723/
Abstract

PREMISE OF THE STUDY

(Hamamelidaceae) is a Tertiary relict species endemic to southern China. Polymorphic microsatellite markers were developed to reveal the genetic diversity of this species.

METHODS AND RESULTS

The genome of was sequenced and de novo assembled into 642,351 contigs. A total of 72,097 paired primers were successfully designed from 80,282 simple sequence repeat (SSR) markers identified in 63,419 contigs. PCR amplification showed that 96 of the 136 synthesized primers could be successfully amplified, and 22 demonstrated polymorphism. The mean number of alleles, levels of observed heterozygosity, and levels of expected heterozygosity were 4.602 ± 0.140, 0.632 ± 0.020, and 0.696 ± 0.010, respectively. The majority of the 96 primer pairs could be amplified in at least one other Hamamelidaceae species, including (60), (39), (24), and (24).

CONCLUSIONS

These microsatellite loci provide abundant genomic SSR markers to evaluate genetic diversity of this woody ornamental plant.

摘要

研究前提

（金缕梅科）是中国南方特有的第三纪残遗物种。开发了多态性微卫星标记以揭示该物种的遗传多样性。

方法与结果

对（该物种）的基因组进行测序并从头组装成642,351个重叠群。从63,419个重叠群中鉴定出的80,282个简单序列重复（SSR）标记中成功设计了总共72,097对引物。PCR扩增表明，136个合成引物中有96个能够成功扩增，22个表现出多态性。等位基因的平均数、观察到的杂合度水平和预期的杂合度水平分别为4.602±0.140、0.632±0.020和0.696±0.010。96对引物中的大多数至少能在其他一种金缕梅科物种中扩增，包括（具体物种1）（60种）、（具体物种2）（39种）、（具体物种3）（24种）和（具体物种4）（24种）。

结论

这些微卫星位点提供了丰富的基因组SSR标记，用于评估这种木本观赏植物的遗传多样性。