用于榕属植物和桑科其他类群进化研究的外显子引物内含子交叉（EPIC）标记。
Exon-primed intron-crossing (EPIC) markers for evolutionary studies of Ficus and other taxa in the fig family (Moraceae).
机构信息
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, Hubei, People's Republic of China ; Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, Michigan 48109-1048 USA.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai, People's Republic of China.
出版信息
Appl Plant Sci. 2013 Oct 4;1(10). doi: 10.3732/apps.1300037. eCollection 2013 Oct.
PREMISE OF THE STUDY
The genus Ficus (fig trees) comprises ca. 750 species of trees, vines, and stranglers found in tropical forests throughout the world. Fig trees are keystone species in many tropical forests, and their relationship with host-specific wasp pollinators has received much attention, although many questions remain unresolved regarding the levels of host specificity, cospeciation, and the role of hybridization in fig and wasp speciation. We developed exon-primed intron-crossing (EPIC) markers to obtain phylogenetic resolution needed to address these questions. •
METHODS AND RESULTS
Expressed sequence tags (ESTs) from F. elastica were compared to Arabidopsis and Populus genomes to locate introns and to design primers in flanking exons. Primer pairs for 80 EPIC markers were tested in samples from divergent clades within Ficus and the outgroup Poulsenia (Moraceae). •
CONCLUSIONS
Thirty-one EPIC markers were successfully sequenced across Ficus, and 29 of the markers also amplified in Poulsenia, indicating broad transferability within Moraceae. All of the EPIC markers were polymorphic and showed levels of polymorphism similar to that of the widely used internal transcribed spacer (ITS).
研究前提
榕属（榕属）植物包括约 750 种树木、藤本植物和在世界各地的热带森林中发现的绞杀植物。榕属植物是许多热带森林中的关键物种，它们与宿主特异性黄蜂传粉者的关系受到了广泛关注，尽管关于宿主特异性、共进化和杂交在榕属和黄蜂属物种形成中的作用，仍有许多问题尚未解决。我们开发了外显子引物交叉（EPIC）标记，以获得解决这些问题所需的系统发育分辨率。
方法和结果
将 F. elastica 的表达序列标签（EST）与拟南芥和杨树基因组进行比较，以定位内含子并在侧翼外显子中设计引物。在榕属和外群 Poulsenia（桑科）内不同分支的样本中测试了 80 对 EPIC 标记的引物。
结论
在榕属中成功地对 31 个 EPIC 标记进行了测序，其中 29 个标记也在 Poulsenia 中扩增，表明在桑科内具有广泛的可转移性。所有的 EPIC 标记都是多态的，其多态性水平与广泛使用的内部转录间隔区（ITS）相似。
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