濒危物种密叶红豆杉（红豆杉科）微卫星位点的开发和特征描述。
Development and characterization of microsatellite loci in the endangered species Taxus wallichiana (Taxaceae).
机构信息
Central Department of Botany, Tribhuvan University, Kathmandu, Nepal ; Swiss Federal Research Institute WSL, Zuercherstrasse 111, CH-8903 Birmensdorf, Switzerland.
Swiss Federal Research Institute WSL, Zuercherstrasse 111, CH-8903 Birmensdorf, Switzerland.
出版信息
Appl Plant Sci. 2013 Feb 27;1(3). doi: 10.3732/apps.1200281. eCollection 2013 Mar.
PREMISE OF THE STUDY
Microsatellite primers were developed in the endangered tree species Taxus wallichiana from Nepal to investigate regional genetic differentiation, local genetic diversity, and gene flow for the conservation of this species under climate- and land-use change scenarios in mountain regions of Nepal. •
METHODS AND RESULTS
We developed 10 highly polymorphic microsatellite markers from 454 DNA sequencing. Characterization of the new microsatellite loci was done in 99 individuals collected from three valleys with different climatic regimes. The number of alleles per locus varied from four to 12. Observed heterozygosity of populations, averaged across loci, ranged from 0.30 to 0.59. •
CONCLUSIONS
The new markers provided by this study will substantially increase the resolution for detailed studies in phylogeography, population genetics, and parentage analysis.
研究前提
从尼泊尔濒危树种红豆杉中开发出微卫星引物，以调查该物种在尼泊尔山区气候和土地利用变化情景下的区域遗传分化、局部遗传多样性和基因流，用于该物种的保护。
方法与结果
我们从 454 个 DNA 测序中开发了 10 个高度多态性的微卫星标记。从三个具有不同气候制度的山谷中采集的 99 个个体对新的微卫星位点进行了特征描述。每个位点的等位基因数从 4 到 12 不等。平均每个位点的观察杂合度在 0.30 到 0.59 之间。
结论
本研究提供的新标记将大大提高在系统地理学、种群遗传学和亲子分析中进行详细研究的分辨率。
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