开发和鉴定野牡丹科直管野牡丹微卫星标记。
Development and characterization of microsatellite markers for Melastoma dodecandrum (Melastomataceae).
机构信息
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
Guangzhou Institute of Landscape Gardening, Guangzhou 510405, People's Republic of China.
出版信息
Appl Plant Sci. 2013 Feb 27;1(3). doi: 10.3732/apps.1200294. eCollection 2013 Mar.
PREMISE OF THE STUDY
Microsatellite markers were developed for Melastoma dodecandrum to investigate the genetic diversity of this species and to detect hybridization and introgression in Melastoma. •
METHODS AND RESULTS
Fourteen microsatellite loci were characterized by screening primers developed using two simple sequence repeat (SSR)-enriched libraries. Based on the genotyping of two natural populations, 13 loci were polymorphic and the number of alleles per locus ranged from two to 15. The observed and expected heterozygosities for the 13 loci ranged from 0.235 to 0.941 and 0.219 to 0.922, respectively. Cross-species amplification was successful for all 14 loci in each of two congeneric species, M. candidum and M. sanguineum. •
CONCLUSIONS
These polymorphic SSR markers could be used as multilocus molecular makers to study the population genetics of M. dodecandrum, as well as hybridization and introgression among Melastoma species.
研究前提
为了研究该物种的遗传多样性，并检测 Melastoma 属中的杂交和基因渐渗，我们开发了用于十二卷Melastoma 的微卫星标记。
方法和结果
通过筛选使用两个简单序列重复（SSR）富集文库开发的引物，对 14 个微卫星位点进行了表征。基于对两个自然种群的基因分型，13 个位点具有多态性，每个位点的等位基因数范围为 2 至 15。13 个位点的观测和预期杂合度分别为 0.235 至 0.941 和 0.219 至 0.922。在两个同属物种，M. candidum 和 M. sanguineum 中，所有 14 个位点的种间扩增均成功。
结论
这些多态性 SSR 标记可作为研究十二卷 Melastoma 种群遗传学以及 Melastoma 属间杂交和基因渐渗的多位点分子标记。
Zotero 插件