针对极度濒危的四方锦香草变种四方锦香草（野牡丹科）开发的20种新型多态性微卫星引物。
Twenty novel polymorphic microsatellite primers in the critically endangered Melastoma tetramerum var. tetramerum (Melastomataceae).
作者信息
Narita Ayu, Izuno Ayako, Komaki Yoshiteru, Tanaka Takefumi, Murata Jin, Isagi Yuji
机构信息
Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
Botanical Gardens, Graduate School of Science, University of Tokyo, 3-7-1 Hakusan, Bunkyo-ku, Tokyo 112-0001, Japan.
出版信息
Appl Plant Sci. 2016 Sep 15;4(9). doi: 10.3732/apps.1600053. eCollection 2016 Sep.
PREMISE OF THE STUDY
Microsatellite markers were identified for Melastoma tetramerum var. tetramerum (Melastomataceae), a critically endangered shrub endemic to the Bonin Islands, to reveal genetic characteristics in wild and restored populations.
METHODS AND RESULTS
Using next-generation sequencing, 27 microsatellite markers were identified. Twenty of these markers were polymorphic in M. tetramerum var. tetramerum, with two to nine alleles per locus and expected heterozygosity ranging from 0.10 to 0.71. Among the 20 polymorphic markers, 15 were applicable to other closely related taxa, namely M. tetramerum var. pentapetalum, M. candidum var. candidum, and M. candidum var. alessandrense.
CONCLUSIONS
These markers can be potentially useful to investigate the genetic diversity, population genetic structure, and reproductive ecology of M. tetramerum var. tetramerum as well as of the three related taxa to provide appropriate genetic information for conservation.
研究前提
为濒危的小笠原群岛特有灌木四花野牡丹（野牡丹科）鉴定微卫星标记，以揭示野生和恢复种群的遗传特征。
方法与结果
利用二代测序技术，鉴定出27个微卫星标记。其中20个标记在四花野牡丹中具有多态性，每个位点有2至9个等位基因，期望杂合度范围为0.10至0.71。在这20个多态性标记中，15个适用于其他近缘类群，即五瓣野牡丹、野牡丹原变种和阿里山野牡丹。
结论
这些标记对于研究四花野牡丹以及三个相关类群的遗传多样性、种群遗传结构和繁殖生态学可能具有潜在用途，可为保护提供适当的遗传信息。
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