利用 Shotgun 焦磷酸测序技术开发和多重扩增西洋杉（柏科）的 SSR 标记。
Development and multiplexed amplification of SSR markers for Thuja occidentalis (Cupressaceae) using shotgun pyrosequencing.
机构信息
Chaire industrielle CRSNG-UQAT-UQAM en Aménagement Forestier Durable, Institut de recherche sur les forêts, Université du Québec en Abitibi-Témiscamingue, 445 Boulevard de l'Université, Rouyn-Noranda, Québec J9X 5E4, Canada ; Northwest A&F University, 3 Taicheng Road, Yangling, Shaanxi 712100, People's Republic of China.
Chaire industrielle CRSNG-UQAT-UQAM en Aménagement Forestier Durable, Institut de recherche sur les forêts, Université du Québec en Abitibi-Témiscamingue, 445 Boulevard de l'Université, Rouyn-Noranda, Québec J9X 5E4, Canada.
出版信息
Appl Plant Sci. 2013 Apr 12;1(5). doi: 10.3732/apps.1200427. eCollection 2013 May.
PREMISE OF THE STUDY
Sixteen novel, polymorphic, multiplexed microsatellite loci were developed for eastern white cedar (Thuja occidentalis) using simple sequence repeat (SSR)-enriched shotgun pyrosequencing. •
METHODS AND RESULTS
Sixteen loci were tested on a panel of 24 individuals from different populations. The number of observed alleles ranged from four to 22. Four sets of multiplex PCR for the 16 loci were then carried out on 60 individuals of two populations from islands of FERLD Duparquet Forest, Canada. Mean number of alleles, observed heterozygosity, and expected heterozygosity were respectively 5.75, 0.594, and 0.574 for Island 58, and 5.50, 0.704, and 0.624 for Island 134. •
CONCLUSIONS
Four sets of multiplex microsatellite loci can be used for future genetic studies, which includes investigating genetic diversity and structure, and fragmentation and regeneration studies.
研究前提
使用简单重复序列（SSR）富集焦磷酸测序技术，为东方白松（Thuja occidentalis）开发了 16 个新的、多态性的、多重微卫星位点。
方法和结果
在来自不同种群的 24 个个体的小组中测试了 16 个位点。观察到的等位基因数从 4 到 22 不等。然后，在来自加拿大 FERLD Duparquet 森林岛屿的两个种群的 60 个个体上进行了 16 个位点的四套多重 PCR。对于 58 号岛，平均等位基因数、观察杂合度和期望杂合度分别为 5.75、0.594 和 0.574，对于 134 号岛，分别为 5.50、0.704 和 0.624。
结论
四套多重微卫星位点可用于未来的遗传研究，包括遗传多样性和结构、片段化和再生研究。
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