鉴定豚草（荨麻科）遗传多样性和城市景观结构的微卫星引物。
Microsatellite primers in Parietaria judaica (Urticaceae) to assess genetic diversity and structure in urban landscapes.
机构信息
Aix Marseille Université, Institut de recherche pour le développement (IRD), Laboratoire Population Environnement Développement (LPED) UMR_D151, 13331 Marseille, France.
Aix Marseille Université, Centre national de la recherche scientifique (CNRS), IRD, Univ. Avignon, IMBE-UMR7263, 13331 Marseille CEDEX 3, France.
出版信息
Appl Plant Sci. 2014 Sep 9;2(9). doi: 10.3732/apps.1400036. eCollection 2014 Sep.
PREMISE OF THE STUDY
Urbanization is one of the main factors contributing to loss of genetic diversity, as the resulting landscape fragmentation and habitat loss induce species isolation. However, studies of genetic structure and diversity in urbanized landscapes are still rare. We characterized microsatellite primers for Parietaria judaica to study this environment. •
METHODS AND RESULTS
Eleven microsatellite loci from P. judaica, an urban plant, were isolated using shotgun pyrosequencing, and the simple sequence repeat (SSR) markers were screened in 20 individuals of P. judaica. The loci were tested on 166 individuals from three populations in different cities. The number of alleles ranged from two to 19, and expected and observed heterozygosity values ranged from 0.019 to 0.912 and 0.019 to 0.448, respectively. •
CONCLUSIONS
The markers amplified well in the species and will be useful for examining genetic diversity and population genetic structure in this urban plant.
研究前提
城市化是导致遗传多样性丧失的主要因素之一，因为由此产生的景观破碎化和栖息地丧失会导致物种隔离。然而，关于城市化景观中的遗传结构和多样性的研究仍然很少。我们为研究这种环境而表征了豚草属植物的微卫星引物。
方法和结果
使用 shotgun 焦磷酸测序从豚草属植物中分离出 11 个微卫星位点，该植物是一种城市植物，并用 20 株豚草属植物的个体进行了简单重复序列 (SSR) 标记的筛选。这些位点在来自三个不同城市的三个种群的 166 个个体中进行了测试。等位基因数从 2 到 19 不等，期望和观察杂合度值分别从 0.019 到 0.912 和 0.019 到 0.448。
结论
这些标记在物种中扩增良好，将有助于研究这种城市植物的遗传多样性和种群遗传结构。
Zotero 插件