柏科植物EST-SSR标记的开发与验证
Development and validation of EST-SSR markers for (Cupressaceae).
作者信息
Ding Mingyan, Meng Kaikai, Fan Qiang, Tan Weizheng, Liao Wenbo, Chen Sufang
机构信息
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of China; Shunde Polytechnic, Foshan 528300, People's Republic of China.
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
出版信息
Appl Plant Sci. 2017 Mar 10;5(3). doi: 10.3732/apps.1600152. eCollection 2017 Mar.
PREMISE OF THE STUDY
(Cupressaceae) is a Tertiary relict evergreen conifer of the monotypic genus . Polymorphic microsatellite markers were developed to investigate its genetic diversity and population structure.
METHODS AND RESULTS
RNA transcripts of were sequenced and de novo assembled into 85,818 unigenes, and 1892 simple sequence repeat (SSR) markers were detected from the unigenes. A total of 273 expressed sequence tag-SSR primer pairs were designed and tested, and 129 successfully amplified. Eleven displayed clear polymorphisms in . Amplification of these polymorphic primers across three populations of showed the number of alleles per locus ranged from two to seven, and the expected heterozygosity per locus varied from 0.067 to 0.847. All 11 polymorphic primers amplified in , while 10 amplified in , , and .
CONCLUSIONS
These microsatellite markers will be useful in exploring genetic diversity of and other conifer trees.
研究前提
（柏科）是单型属的第三纪残遗常绿针叶树。开发了多态微卫星标记以研究其遗传多样性和种群结构。
方法与结果
对的RNA转录本进行测序并从头组装成85,818个单基因，从这些单基因中检测到1892个简单序列重复（SSR）标记。总共设计并测试了273对表达序列标签-SSR引物，其中129对成功扩增。11对在中显示出明显的多态性。这些多态性引物在的三个种群中的扩增表明，每个位点的等位基因数范围为2至7，每个位点的预期杂合度在0.067至0.847之间变化。所有11对多态性引物在中均能扩增，而10对在、和中能扩增。
结论
这些微卫星标记将有助于探索和其他针叶树的遗传多样性。
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