开发微卫星标记用于研究巴西大西洋森林特有种——马鞭草科假鹰爪属植物（Verbenoxylum reitzii）。
Development of microsatellites for Verbenoxylum reitzii (Verbenaceae), a tree endemic to the Brazilian Atlantic Forest.
机构信息
Programa de Pós-Graduação em Botânica, Universidade Federal do Rio Grande do Sul, Bento Gonçalves 9500, Porto Alegre, Brazil ; Laboratory of Molecular Evolution, Department of Genetics, Universidade Federal do Rio Grande do Sul, PO Box 15053, Porto Alegre, Brazil.
Laboratory of Molecular Evolution, Department of Genetics, Universidade Federal do Rio Grande do Sul, PO Box 15053, Porto Alegre, Brazil.
出版信息
Appl Plant Sci. 2013 Jul 15;1(8). doi: 10.3732/apps.1300005. eCollection 2013 Aug.
PREMISE OF THE STUDY
Microsatellite markers were developed for Verbenoxylum reitzii (Verbenaceae), a tree endemic to the Brazilian Atlantic Forest, to investigate their usefulness in population genetic studies. The loci were tested for cross-amplification in the related genera Recordia and Duranta. •
METHODS AND RESULTS
Eleven polymorphic microsatellite markers were isolated from an enriched library of V. reitzii and characterized. The primers were tested on 60 individuals from three populations of this species. The number of alleles per locus ranged from two to 11, and the observed and expected heterozygosities varied from 0.0 to 1.0 and from 0.088 to 0.758, respectively. Ten loci successfully amplified in R. boliviana and all failed in D. vestita. •
CONCLUSIONS
Our results suggest the usefulness of the microsatellite loci developed here to access genetic variability for phylogeographic and population genetic studies in V. reitzii, which are important for the conservation of this rare species.
研究前提
开发了用于巴西大西洋森林特有树种 Verbenoxylum reitzii（马鞭草科）的微卫星标记，以研究其在种群遗传研究中的有用性。测试了这些基因座在相关属 Recordia 和 Duranta 中的交叉扩增情况。
方法和结果
从 V. reitzii 的富集文库中分离出 11 个多态性微卫星标记，并对其进行了特征描述。这些引物在该物种的三个种群的 60 个个体上进行了测试。每个基因座的等位基因数范围从 2 到 11，观察到的和预期的杂合度分别从 0.0 到 1.0 和从 0.088 到 0.758 不等。10 个基因座在 R. boliviana 中成功扩增，而在 D. vestita 中均未扩增。
结论
我们的结果表明，这里开发的微卫星基因座可用于研究 V. reitzii 的遗传变异性，这对于保护这种稀有物种的系统地理学和种群遗传学研究具有重要意义。
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