含羞草科具刺皮檀微卫星标记的开发与特征分析
Development and characterization of microsatellite markers for Piptadenia gonoacantha (Fabaceae).
作者信息
Grando Carolina, Bajay Miklos M, Bajay Stephanie K, Schwarcz Kaiser D, Campos Jaqueline B, Brancalion Pedro H S, Pinheiro José B, Rodrigues Ricardo R, Souza Anete P, Zucchi Maria I
机构信息
Programa de Pós-graduação em Genética e Biologia Molecular, Universidade Estadual de Campinas, CP 6109, Rua Bertrand Russel, 13083-970 Campinas, São Paulo, Brazil.
Departamento de Genética, Universidade de São Paulo (USP), Av. Pádua Dias 11, 13418-900 Piracicaba, São Paulo, Brazil.
出版信息
Appl Plant Sci. 2015 Feb 9;3(2). doi: 10.3732/apps.1400107. eCollection 2015 Feb.
UNLABELLED
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PREMISE OF THE STUDY
Microsatellite primers were designed for Piptadenia gonoacantha (Fabaceae) and characterized to estimate genetic diversity parameters. The species is a native tree from the Atlantic Forest biome commonly used in forest restoration; it has medicinal potential and the wood is economically useful. •
METHODS AND RESULTS
Twenty-eight microsatellite loci were identified from an enriched genomic library. Fifteen loci resulted in successful amplifications and were characterized in a natural population of 94 individuals. Twelve loci were polymorphic, with allele numbers ranging from three to 15 per locus, and expected and observed heterozygosities ranging from 0.2142 to 0.8325 and 0.190 to 0.769, respectively. •
CONCLUSIONS
The developed markers will be used in further studies of population genetics of P. gonoacantha, aimed at conservation and management of the species in natural populations and in forest restoration projects.
未标注
研究前提：为具腺匹珍蝶（豆科）设计了微卫星引物，并对其进行特征分析以估计遗传多样性参数。该物种是一种原产于大西洋森林生物群落的本土树木，常用于森林恢复；它具有药用潜力，木材具有经济价值。
方法与结果：从一个富集基因组文库中鉴定出28个微卫星位点。15个位点成功扩增，并在94个个体的自然种群中进行了特征分析。12个位点具有多态性，每个位点的等位基因数从3到15不等，预期杂合度和观察杂合度分别为0.2142至0.8325和0.190至0.769。
结论：所开发的标记将用于具腺匹珍蝶种群遗传学的进一步研究，旨在保护和管理自然种群中的该物种以及森林恢复项目。
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