Institute of Agriculture and Environment, Massey University, Palmerston North 4442, New Zealand.
Landcare Research, Lincoln 7640, New Zealand.
Appl Plant Sci. 2014 Feb 11;2(3). doi: 10.3732/apps.1300081. eCollection 2014 Mar.
Genus-specific microsatellite markers were developed for Sophora for population genetic and systematic studies of the group in New Zealand, and potentially elsewhere in the geographic range. •
From sequencing a total genomic DNA library (using Roche 454), we identified and developed 29 polymorphic microsatellite markers for S. microphylla and S. chathamica. We tested 12 of these markers on 14 S. chathamica individuals and four S. microphylla populations. All loci amplified in both species and species-specific alleles occurred at seven loci. In S. microphylla populations, the observed and expected heterozygosities ranged from 0.000-0.960 and 0.000-0.908, respectively, with alleles per locus ranging from seven to 23. •
The developed markers will be valuable in studies of phylogenetics, population structure, mating system, and selection of provenances for restoration projects.
为了进行群体遗传和系统研究，我们为新西兰的槐属植物开发了种特异性微卫星标记，这些标记也可能在地理分布范围内的其他地方使用。
通过对总基因组 DNA 文库（使用罗氏 454 测序）进行测序，我们鉴定并开发了 29 个用于小冠花槐和查塔姆槐的多态性微卫星标记。我们在 14 个查塔姆槐个体和 4 个小冠花槐群体上测试了其中的 12 个标记。所有标记在两个物种中都能扩增，且在 7 个位点上出现了种特异性等位基因。在小冠花槐群体中，观察到的和预期的杂合度分别在 0.000-0.960 和 0.000-0.908 之间，每个位点的等位基因数在 7 到 23 之间。
开发的标记将在系统发育、种群结构、交配系统以及用于恢复项目的种源选择方面的研究中具有重要价值。
