Belletti Daniela, Vandelli Maria Angela, Tonelli Massimo, Zapparoli Mauro, Forni Flavio, Tosi Giovanni, Ruozi Barbara
Department of Life Sciences , University of Modena and Reggio Emilia, Via Campi 183, Modena , Italy and.
J Liposome Res. 2015;25(2):150-6. doi: 10.3109/08982104.2014.956221. Epub 2014 Sep 9.
The development of smart delivery systems able to deliver and target a drug to the site of action is one of the major challenges in the field of pharmaceutical technology. The surface modification of nanocarriers, such as liposomes, is widely investigated either for increasing the blood circulation time (by pegylation) or for interacting with specific tissues or cells (by conjugation of a selective ligand as a monoclonal antibody, mAb). Microscopical analysis thereby is a useful approach to evaluate the morphology and the size owing to resolution and versatility in defining either surface modification or the architecture and the internal structure of liposomes. This contribution aims to connect the outputs obtained by transmission electron (TEM) and atomic force (AFM) microscopical techniques for identifying the modifications on the liposomal surface. To reach this objective, we prepared liposomes applying two different pegylation technologies and further modifying the surface by mAb conjugation. This work demonstrates the feasibility to apply the combined approach (TEM and AFM analysis) in the evaluation of the efficacy of a surface engineering process.
开发能够将药物输送并靶向作用部位的智能给药系统是制药技术领域的主要挑战之一。纳米载体（如脂质体）的表面修饰被广泛研究，其目的要么是延长血液循环时间（通过聚乙二醇化），要么是与特定组织或细胞相互作用（通过偶联选择性配体如单克隆抗体，mAb）。因此，显微镜分析是评估形态和尺寸的有用方法，这是由于其在定义脂质体的表面修饰或结构及内部结构方面具有分辨率和多功能性。本论文旨在将通过透射电子显微镜（TEM）和原子力显微镜（AFM）技术获得的结果联系起来，以识别脂质体表面的修饰。为实现这一目标，我们制备了采用两种不同聚乙二醇化技术的脂质体，并通过mAb偶联进一步修饰其表面。这项工作证明了应用联合方法（TEM和AFM分析）评估表面工程过程效果的可行性。
