Department of General, Thoracic, Vascular and Transplantation Surgery, University of Rostock, Schillingallee 35, D-18057 Rostock, Germany.
Institute for Biomedical Engineering, University of Rostock, Friedrich-Barnewitz-Str. 4, D-18119 Rostock, Germany.
Acta Biomater. 2014 Dec;10(12):5081-5089. doi: 10.1016/j.actbio.2014.09.002. Epub 2014 Sep 7.
As it has been demonstrated that bioactive substances can be delivered locally using coated surgical suture materials, the authors developed a vascular endothelial growth factor (VEGF)-releasing suture material that should promote vascularization and potentially wound healing. In this context, the study focused on the characterization of the developed suture material and the verification of its biological activity, as well as establishing a coating process that allows reproducible and stable coating of a commercially available polydioxanone suture material with poly(l-lactide) (PLLA) and 0.1μg and 1.0μg VEGF. The in vitro VEGF release kinetics was studied using a Sandwich ELISA. The biological activity of the released VEGF was investigated in vitro using human umbilical vein endothelial cells. The potential of the VEGF-releasing suture material was also studied in vivo 5days after implantation in the hind limb of Wistar rats, when the histological findings were analyzed. The essential results, enhanced cell viability in vitro as well as significantly increased vascularization in vivo, were achieved using PLLA/1.0μg VEGF-coated suture material. Furthermore, ELISA measurements revealed a high reproducibility of the VEGF release behavior. Based on the results achieved regarding the dose-effect relationship of VEGF, the stability during its processing and the release behavior, it can be predicted that a bioactive suture material would be successful in later in vivo studies. Therefore, this knowledge could be the basis for future studies, where bioactive substances with different modes of action are combined for targeted, overall enhancement of wound healing.
由于已经证明可以使用涂层外科缝线材料局部递送生物活性物质，作者开发了一种血管内皮生长因子（VEGF）释放缝线材料，该材料应能促进血管生成并潜在促进伤口愈合。在这方面，该研究集中于开发缝线材料的特性和生物活性的验证，以及建立一种涂层工艺，该工艺允许用聚（L-丙交酯）（PLLA）和 0.1μg 和 1.0μg VEGF 可重复且稳定地涂覆市售的聚二恶烷酮缝线材料。使用 Sandwich ELISA 研究了体外 VEGF 释放动力学。使用人脐静脉内皮细胞在体外研究了释放的 VEGF 的生物活性。在植入 Wistar 大鼠后肢 5 天后，还研究了 VEGF 释放缝线材料的体内潜力，分析了组织学发现。使用 PLLA/1.0μg VEGF 涂层缝线材料可实现体外细胞活力增强以及体内血管化明显增加等重要结果。此外，ELISA 测量显示 VEGF 释放行为具有很高的重现性。基于 VEGF 剂量效应关系、处理过程中的稳定性以及释放行为方面的结果，可以预测生物活性缝线材料在以后的体内研究中会取得成功。因此，这些知识可以为未来的研究提供基础，在这些研究中，可以将具有不同作用模式的生物活性物质结合起来，有针对性地全面增强伤口愈合。
