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Transcriptomic Response Pathways of Yeast to Crucial Polyphenolic Acids in Rosmarinus Acid Biosynthesis.酵母对迷迭香酸生物合成中关键多酚酸的转录组反应途径
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Reinforced NADPH regeneration in engineered Saccharomyces cerevisiae enhances rosmarinic acid production.工程化酿酒酵母中增强的NADPH再生可提高迷迭香酸的产量。
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Cofactor recycling strategies for secondary metabolite production in cell-free protein expression systems.无细胞蛋白质表达系统中次生代谢产物生产的辅因子循环策略。
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本文引用的文献

1
Cytochrome P450-mediated metabolic engineering: current progress and future challenges.细胞色素P450介导的代谢工程：当前进展与未来挑战。
Curr Opin Plant Biol. 2014 Jun;19:27-34. doi: 10.1016/j.pbi.2014.03.004. Epub 2014 Apr 5.
2
Extending shikimate pathway for the production of muconic acid and its precursor salicylic acid in Escherichia coli.在大肠杆菌中扩展莽草酸途径以生产粘康酸及其前体水杨酸。
Metab Eng. 2014 May;23:62-9. doi: 10.1016/j.ymben.2014.02.009. Epub 2014 Feb 25.
3
Rosmarinic acid ameliorates PTSD-like symptoms in a rat model and promotes cell proliferation in the hippocampus.迷迭香酸可改善大鼠模型中的创伤后应激障碍样症状，并促进海马体中的细胞增殖。
Prog Neuropsychopharmacol Biol Psychiatry. 2014 Jun 3;51:16-22. doi: 10.1016/j.pnpbp.2014.01.002. Epub 2014 Jan 11.
4
Metabolic engineering of Escherichia coli to optimize melanin synthesis from glucose.大肠杆菌的代谢工程优化葡萄糖合成黑色素。
Microb Cell Fact. 2013 Nov 13;12:108. doi: 10.1186/1475-2859-12-108.
5
Neuroprotective effects of rosmarinic acid on ciguatoxin in primary human neurons.迷迭香酸对原代人神经元中海藻毒素的神经保护作用。
Neurotox Res. 2014 Feb;25(2):226-34. doi: 10.1007/s12640-013-9429-9. Epub 2013 Oct 5.
6
Production of hydroxycinnamoyl anthranilates from glucose in Escherichia coli.在大肠杆菌中从葡萄糖生产羟基肉桂酰基邻氨基苯甲酸酯。
Microb Cell Fact. 2013 Jun 28;12:62. doi: 10.1186/1475-2859-12-62.
7
Metabolic engineering of Escherichia coli for production of salvianic acid A via an artificial biosynthetic pathway.大肠杆菌通过人工生物合成途径生产丹酚酸 A 的代谢工程改造。
Metab Eng. 2013 Sep;19:79-87. doi: 10.1016/j.ymben.2013.06.001. Epub 2013 Jun 14.
8
Rosmarinic acid ameliorates depressive-like behaviors in a rat model of CUS and Up-regulates BDNF levels in the hippocampus and hippocampal-derived astrocytes.迷迭香酸可改善慢性不可预知应激模型大鼠的抑郁样行为，并上调海马和海马来源星形胶质细胞中的脑源性神经营养因子水平。
Neurochem Res. 2013 Sep;38(9):1828-37. doi: 10.1007/s11064-013-1088-y. Epub 2013 Jun 12.
9
Production of hydroxycinnamoyl-shikimates and chlorogenic acid in Escherichia coli: production of hydroxycinnamic acid conjugates.在大肠杆菌中合成羟基肉桂酰基-莽草酸和绿原酸：羟基肉桂酸缀合物的合成。
Microb Cell Fact. 2013 Feb 5;12:15. doi: 10.1186/1475-2859-12-15.
10
Artificial biosynthesis of phenylpropanoic acids in a tyrosine overproducing Escherichia coli strain.在一个酪氨酸过量生产的大肠杆菌菌株中人工合成苯丙酸。
Microb Cell Fact. 2012 Dec 3;11:153. doi: 10.1186/1475-2859-11-153.

在大肠杆菌中构建用于迷迭香酸从头生物合成的嵌合生物合成途径。

Construction of a chimeric biosynthetic pathway for the de novo biosynthesis of rosmarinic acid in Escherichia coli.

作者信息

Bloch Sarah E, Schmidt-Dannert Claudia

机构信息

Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, 1479 Gortner Avenue, St. Paul, MN 55108 (USA).

出版信息

Chembiochem. 2014 Nov 3;15(16):2393-401. doi: 10.1002/cbic.201402275. Epub 2014 Sep 9.

DOI:10.1002/cbic.201402275
PMID:25205019
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4264569/
Abstract

Hydroxycinnamic acid esters (HCEs) are widely-distributed phenylpropanoid-derived plant natural products. Rosmarinic acid (RA), the most well-known HCE, shows promise as a treatment for cancer and neurological disorders. In contrast to extraction from plant material or plant cell culture, microbial production of HCEs could be a sustainable, controlled means of production. Through the overexpression of a six-enzyme chimeric bacterial and plant pathway, we show the de novo biosynthesis of RA, and the related HCE isorinic acid (IA), in Escherichia coli. Probing the pathway through precursor supplementation showed several potential pathway bottlenecks. We demonstrated HCE biosynthesis using three plant rosmarinic acid synthase (RAS) orthologues, which exhibited different levels of HCE biosynthesis but produced the same ratio of IA to RA. This work serves as a proof-of-concept for a microbial production platform for HCEs by using a modular biosynthetic approach to access diverse natural and non-natural HCEs.

摘要

羟基肉桂酸酯（HCEs）是广泛分布的源自苯丙烷类的植物天然产物。迷迭香酸（RA）是最著名的HCE，有望用于治疗癌症和神经疾病。与从植物材料或植物细胞培养物中提取不同，微生物生产HCEs可能是一种可持续、可控的生产方式。通过过表达一条由六种酶组成的嵌合细菌和植物途径，我们展示了在大肠杆菌中从头生物合成RA以及相关的HCE异迷迭香酸（IA）。通过前体补充对该途径进行探究，发现了几个潜在的途径瓶颈。我们使用三种植物迷迭香酸合酶（RAS）直系同源物证明了HCE的生物合成，它们表现出不同水平的HCE生物合成，但IA与RA的比例相同。这项工作通过使用模块化生物合成方法来获取各种天然和非天然的HCEs，为HCEs的微生物生产平台提供了概念验证。