Department of Gastroenterology, University of Modena and Reggio Emilia, Modena, Italy; Parco Tecnologico Padano, via Einstein, Lodi, Italia.
J Cell Physiol. 2015 Apr;230(4):821-30. doi: 10.1002/jcp.24810.
The Coiled-Coil Domain Containing 80 (CCDC80) gene has been identified as strongly induced in rat thyroid PC CL3 cells immortalized by the adenoviral E1A gene. In human, CCDC80 is a potential oncosoppressor due to its down-regulation in several tumor cell lines and tissues and it is expressed in almost all tissues. CCDC80 has homologous in mouse, chicken, and zebrafish. We cloned the zebrafish ccdc80 and analyzed its expression and function during embryonic development. The in-silico translated zebrafish protein shares high similarity with its mammalian homologous, with nuclear localization signals and a signal peptide. Gene expression analysis demonstrates that zebrafish ccdc80 is maternally and zygotically expressed throughout the development. In particular, ccdc80 is strongly expressed in the notochord and it is under the regulation of the Hedgehog pathway. In this work we investigated the functional effects of ccdc80-loss-of-function during embryonic development and verified its interaction with gadd45β2 in somitogenesis.
卷曲螺旋结构域包含 80（CCDC80）基因已被鉴定为在大鼠甲状腺 PC CL3 细胞中强烈诱导，这些细胞被腺病毒 E1A 基因永生化。在人类中，CCDC80 是一种潜在的抑癌基因，因为它在几种肿瘤细胞系和组织中下调，并且几乎在所有组织中表达。CCDC80 在小鼠、鸡和斑马鱼中有同源物。我们克隆了斑马鱼 ccdc80，并分析了其在胚胎发育过程中的表达和功能。计算机翻译的斑马鱼蛋白与哺乳动物同源物具有高度相似性，具有核定位信号和信号肽。基因表达分析表明，斑马鱼 ccdc80 在整个发育过程中均呈母系和合子表达。特别是，ccdc80 在脊索中强烈表达，并受 Hedgehog 途径的调节。在这项工作中，我们研究了 ccdc80 功能丧失在胚胎发育过程中的功能影响，并验证了它在体节发生过程中与 gadd45β2 的相互作用。
