Department of Basic Theory of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510060, Guangdong Province, China.
Department of Traditional Chinese Medicine, Luzhou Medicine College, Luzhou 646000, Sichuang Province, China.
Neural Regen Res. 2013 Apr 25;8(12):1091-102. doi: 10.3969/j.issn.1673-5374.2013.12.004.
The traditional Chinese medicine Jiaweisinisan has antidepressant effects, and can inhibit hypothalamus-pituitary-adrenal gland axis hyperactivity in stress-induced depression. In this study, rat hippocampal neural precursor cells were cultured in serum-free medium in vitro and a stress damage model was established with 120 μM corticosterone. Cells were treated with 10% (v/v) Jiaweisinisan drug-containing serum and the corticosterone antagonist RU38486. Results of the 3-(4,5-dimethylthiazol-2-yl)-3,5-di-phenytetrazoliumromide assay showed that both Jiaweisinisan drug-containing serum and RU38486 promoted the proliferation of neural precursor cells after corticosterone exposure. Immunofluorescence detection showed that after Jiaweisinisan drug-containing serum and RU38486 treatment, the 5-bromo-2-deoxyuridine/terminal deoxynucleotidyl transferase dUTP nick end labeling ratio in hippocampal neural precursor cells significantly increased, and the apoptotic rates of glial cells reduced, and neuron-like cell differentiation from neural precursor cells significantly increased. Our experimental findings indicate that Jiaweisinisan promotes hippocampal neurogenesis after stress damage.
加味四逆散具有抗抑郁作用，能抑制应激诱导抑郁模型中下丘脑-垂体-肾上腺轴的过度兴奋。本研究采用无血清培养方法体外培养大鼠海马神经前体细胞，用 120μM 皮质酮建立应激损伤模型。用含 10%（v/v）加味四逆散血清和皮质酮拮抗剂 RU38486 处理细胞。3-(4,5-二甲基噻唑-2-基)-3,5-二苯基四氮唑溴盐（MTT）比色法结果表明，加味四逆散含药血清和 RU38486 均可促进皮质酮暴露后的神经前体细胞增殖。免疫荧光检测显示，加味四逆散含药血清和 RU38486 处理后，海马神经前体细胞中的 5-溴-2-脱氧尿苷/末端脱氧核苷酸转移酶 dUTP 缺口末端标记比率显著增加，神经胶质细胞的凋亡率降低，神经前体细胞向神经元样细胞的分化明显增加。我们的实验结果表明，加味四逆散能促进应激损伤后的海马神经发生。
