Department of Ophthalmology, First Affiliated Hospital, Harbin Medical University, Harbin 200120, Heilongjiang Province, China ; Department of Ophthalmology, Shanghai East Hospital Affiliated to Tongji University, Shanghai 200120, China.
Department of Histology and Embryology, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China.
Neural Regen Res. 2013 Jul 5;8(19):1783-92. doi: 10.3969/j.issn.1673-5374.2013.19.006.
Human Wharton's jelly mesenchymal stem cells were isolated from fetal umbilical cord. Cells were cultured in serum-free neural stem cell-conditioned medium or neural stem cell-conditioned medium supplemented with Dkk-1, a Wnt/β catenin pathway antagonist, and LeftyA, a Nodal signaling pathway antagonist to induce differentiation into retinal progenitor cells. Inverted microscopy showed that after induction, the spindle-shaped or fibroblast-like Wharton's jelly mesenchymal stem cells changed into bulbous cells with numerous processes. Immunofluorescent cytochemical ing and reverse-transcription PCR showed positive expression of retinal progenitor cell markers, Pax6 and Rx, as well as weakly down-regulated nestin expression. These results demonstrate that Wharton's jelly mesenchymal stem cells are capable of differentiating into retinal progenitor cells in vitro.
人脐带华通氏胶间充质干细胞从胎儿脐带中分离得到。将细胞培养在无血清神经干细胞条件培养基或补充有 Wnt/β 连环蛋白通路拮抗剂 Dkk-1 和 Nodal 信号通路拮抗剂 LeftyA 的神经干细胞条件培养基中，以诱导分化为视网膜祖细胞。倒置显微镜下观察到诱导后，呈纺锤形或成纤维细胞样的华通氏胶间充质干细胞变为具有多个突起的球形体。免疫荧光细胞化学和逆转录 PCR 显示视网膜祖细胞标志物 Pax6 和 Rx 呈阳性表达，同时巢蛋白表达水平下调。这些结果表明，人脐带华通氏胶间充质干细胞在体外具有分化为视网膜祖细胞的能力。
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