Horká Marie, Karásek Pavel, Růžička Filip, Dvořáčková Milada, Sittová Martina, Roth Michal
Institute of Analytical Chemistry of the ASCR , v. v. i., Veveří 97, 602 00 Brno, Czech Republic.
Anal Chem. 2014 Oct 7;86(19):9701-8. doi: 10.1021/ac502254f. Epub 2014 Sep 23.
Identification and prevention of Staphylococcus aureus-caused infections may benefit from a fast and dependable method to distinguish between the methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) S. aureus strains. The current methods involving polymerase chain reaction and/or other molecular tests are usually laborious and time-consuming. We describe here a fast and low-cost method employing capillary zone electrophoresis (CZE) to distinguish between MRSA and MSSA. The method makes use of a supercritical water-treated fused silica capillary, the inner surface of which has subsequently been modified with (3-glycidyloxypropyl)trimethoxysilane. With optimized proportions of suitable additives to the background electrolyte, a CZE separation of MRSA from MSSA may be completed within 12 min. The cells were baseline-resolved, and resolution was determined to be 3.61. The isoelectric points of MSSA and MRSA were found to be the same for both groups of these strains, pI = 3.4.
识别和预防金黄色葡萄球菌引起的感染可能受益于一种快速可靠的方法，以区分耐甲氧西林金黄色葡萄球菌（MRSA）和甲氧西林敏感金黄色葡萄球菌（MSSA）菌株。目前涉及聚合酶链反应和/或其他分子检测的方法通常费力且耗时。我们在此描述一种采用毛细管区带电泳（CZE）区分MRSA和MSSA的快速低成本方法。该方法使用超临界水处理的熔融石英毛细管，其内壁随后用（3-缩水甘油氧基丙基）三甲氧基硅烷进行了改性。通过向背景电解质中加入优化比例的合适添加剂，可在12分钟内完成MRSA与MSSA的CZE分离。细胞实现了基线分离，分辨率测定为3.61。发现这两组菌株的MSSA和MRSA的等电点相同，pI = 3.4。
