Institute of Analytical Chemistry of the CAS, Veveří 97, 602 00, Brno, Czech Republic.
Department of Microbiology, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
Anal Chim Acta. 2022 Sep 22;1227:340305. doi: 10.1016/j.aca.2022.340305. Epub 2022 Aug 31.
This study describes differentiation of methicillin-resistant Staphylococcus aureus (MRSA) isolates belonging to different genotype groups by the combination of electrophoretic techniques, transient isotachophoresis and micellar electrokinetic chromatography. MRSA isolates were separated in fused silica capillary with roughened inner surface prepared by etching with supercritical water. Separation temperature together with the rinsing procedure of the capillary turned out to be the key factors of successful analysis. The individual genotype groups were baseline-resolved in 40 min. Partial separation of the individual isolates within the groups was also observed. Relative standard deviations of the migration times of the isolate zones ranged from 0.32 to 0.79%. In addition, capability of the developed CE method to concentrate and separate MRSA isolates in clinical samples was proved by the analysis of blood sample.
本研究通过电泳技术、瞬变等电聚焦和胶束电动色谱相结合，对不同基因型组的耐甲氧西林金黄色葡萄球菌（MRSA）分离株进行了区分。MRSA 分离株在经超临界水蚀刻处理的粗糙内表面的熔融石英毛细管中进行分离。分离温度和毛细管的冲洗程序是成功分析的关键因素。在 40 分钟内实现了各个基因型组的基线分离。还观察到了各个组内的个别分离物的部分分离。分离物区带的迁移时间的相对标准偏差范围为 0.32 至 0.79%。此外，通过对血液样本的分析，证明了所开发的 CE 方法在浓缩和分离临床样本中的 MRSA 分离物方面的能力。
