Wang Y, Zhang G-H, Li S-L
Department of Radiation Medicine, Chongqing Medical University, Chongqing, China.
Drug Res (Stuttg). 2015 Jun;65(6):323-6. doi: 10.1055/s-0034-1382077. Epub 2014 Sep 10.
Recent studies reported that cancer stem cells (CSCs) are responsible for treatment failure and tumor recurrence. CSCs have been isolated and characterized in several solid tumors. However, the information regarding the CSCs from nasopharyngeal carcinoma (NPC) is very less. Therefore, in the current study we aimed to isolate and characterize the cancer stem like SP cells from NPC.
We have used FACS based Hoechst 33342 dye exclusion technique to identify the cancer stem like SP cells from NPC. The FACS sorted SP cells are further subjected to drug resistance assay, clonogenic formation assay and immunocytochemistry analysis for the expression of CD44 and ABCG2.
We have identified 3.9% of cancer stem like SP cells from NPC. Upon treatment with verapamil, the percentage of SP cells was reduced to 0.9%. The FACS sorted SP cells showed enhanced expression of CD44 and ABCG2. Further, these SP cells are highly resistant to multi-drugs and high potent of sphere formation when compared to the non-SP cells.
Our data clearly suggest that presence of cancer stem like SP cells from nasopharyngeal carcinoma might responsible for chemotherapeutic drug resistance, tumor recurrence and invasion.
最近的研究报道，癌症干细胞（CSCs）是治疗失败和肿瘤复发的原因。在几种实体瘤中已分离并鉴定出癌症干细胞。然而，关于鼻咽癌（NPC）中癌症干细胞的信息非常少。因此，在本研究中，我们旨在从鼻咽癌中分离并鉴定癌症干细胞样SP细胞。
我们使用基于荧光激活细胞分选（FACS）的Hoechst 33342染料排除技术从鼻咽癌中鉴定癌症干细胞样SP细胞。对通过FACS分选的SP细胞进一步进行耐药性测定、克隆形成测定以及CD44和ABCG2表达的免疫细胞化学分析。
我们从鼻咽癌中鉴定出3.9%的癌症干细胞样SP细胞。用维拉帕米处理后，SP细胞的百分比降至0.9%。通过FACS分选的SP细胞显示出CD44和ABCG2的表达增强。此外，与非SP细胞相比，这些SP细胞对多种药物具有高度抗性且具有很高的成球能力。
我们的数据清楚地表明，鼻咽癌中癌症干细胞样SP细胞的存在可能是化疗耐药、肿瘤复发和侵袭的原因。
