单独激活Cre重组酶可诱导肿瘤完全消退。
Activation of Cre recombinase alone can induce complete tumor regression.
作者信息
Li Yulin, Choi Peter S, Casey Stephanie C, Felsher Dean W
机构信息
Department of Medicine, Division of Oncology, School of Medicine, Stanford University, Stanford, California, United States of America.
出版信息
PLoS One. 2014 Sep 10;9(9):e107589. doi: 10.1371/journal.pone.0107589. eCollection 2014.
The Cre/loxP system is a powerful tool for generating conditional genomic recombination and is often used to examine the mechanistic role of specific genes in tumorigenesis. However, Cre toxicity due to its non-specific endonuclease activity has been a concern. Here, we report that tamoxifen-mediated Cre activation in vivo induced the regression of primary lymphomas in p53-/- mice. Our findings illustrate that Cre activation alone can induce the regression of established tumors.
Cre/loxP系统是用于产生条件性基因组重组的强大工具，常用于研究特定基因在肿瘤发生中的机制作用。然而，由于其非特异性核酸内切酶活性导致的Cre毒性一直是个问题。在此，我们报告在体内他莫昔芬介导的Cre激活可诱导p53基因敲除小鼠原发性淋巴瘤的消退。我们的研究结果表明，仅Cre激活就能诱导已形成肿瘤的消退。
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