Zhang Qian, Fu Shi-Yu, Li Hai-Long, Zhan Huai-Yu
Guang Pu Xue Yu Guang Pu Fen Xi. 2014 Mar;34(3):767-70.
The present paper reported a spectrophotometry for the determination of hydrogen peroxide concentration. It is based on the reaction between ceric sulfate and hydrogen peroxide in an acidic medium, in which orange tetravalent cerium is converted to colorless trivalent cerium that causeds the change in absorbance. According to the quantitative relationship between ceric sulfate and hydrogen peroxide, the hydrogen peroxide concentration can be calculated. The selected conditions were as follows: detection wavelength of 480 nm, H2SO4 concentration of 0.5 mol x L(-1), and reaction time of 3.0 min. The results showed that the method has an excellent measurement precision (RSD = 0.31%) and accuracy (RSD < or = 0.91%) for the quantification of hydrogen peroxide. The present method is simple and rapid, with high detection sensitivity and low cost. It is suitable for use in rapid industrial analyses.
本文报道了一种用于测定过氧化氢浓度的分光光度法。它基于硫酸铈与过氧化氢在酸性介质中的反应，在此反应中橙色的四价铈转化为无色的三价铈，导致吸光度发生变化。根据硫酸铈与过氧化氢之间的定量关系，可以计算出过氧化氢的浓度。所选条件如下：检测波长为480nm，硫酸浓度为0.5mol·L⁻¹，反应时间为3.0分钟。结果表明，该方法在过氧化氢定量分析方面具有出色的测量精密度（相对标准偏差RSD = 0.31%）和准确度（RSD≤0.91%）。本方法简单快速，检测灵敏度高，成本低。适用于快速的工业分析。
